Polyphenols Exhaustive Exercise Mice Myocardial Tissue Calcium Content

Objective:To study the effect of tea polyphenols on the free radical's metabolism, ATPase andtotal calcium content in the acute exhausted rats myocardial tissues. And the result canprovide experiment and theory proof for that tea polyphenols can rivalry myocardiuminjury, sports fatigue and elevate motor ability.Method:Thirteen ICR rats were divided into three groups, namely: Control(C), Exercise (E),TpExercise(TPE). The rats were raised in a clean animal lab in Yangzhou University, theTPE Group were fed with tea polyphenols (300mg/kg/d), the C and E Group were fed withthe same volume of distilled water. Then in the seventh day, E and TPE Group marched anacute exhausted swimming with no weight overloading at the same time. After every rats inthe E and TPE Group were exhausted, instantly they were taken out, then measure the timeof exhausted swimming. Then instantly decapitation and unload heart. At last homogenateto determine MDA,SOD,GSH-Px,T-AOC, Na⁺-K⁺-ATPase,Ca²⁺-ATPase and the totalcalcium content. Use SPSS statistics software to carry on the statistical analysis.Result:(1) Compared with C group, the total calcium content of myocardial tissuessignificantly increased (P＜0.05), the activity of Na⁺-K⁺-ATPase and Ca²⁺-ATPase ofmyocardial tissues remarkably decreased (P＜0.01), the content of MDA of myocardialtissues more significantly went up (P＜0.01), the activity of SOD,GSH-Px of myocardialtissues descended highly significantly (P＜0.01), the ability T-AOC of myocardial tissuesstrikingly decreased (P＜0.05) in E Group. These results indicated that exhaustedswimming could damage the cardiac muscle as the result of the augementation of freeradical, break down the activity of ATPase and evoke the calcium overloading.(2) Compared with E Group, the time of exhausted swimming was significantlylonger(P＜0.05), the total calcium content of myocardial tissues decreasedsignificantly(P＜0.05), the activity of Na⁺-K⁺-ATPase and Ca²⁺-ATPase of myocardialtissues both had a more significantly tendency of ascensus (P＜0.05),the content of MDAof myocardial tissues is more significantly decreased (P＜0.05),the activity of SOD(P＜0.01),GSH-Px (P＜0.05)and the ability T-AOC (P＜0.01) of myocardial tissues wereremarkably increased in TPE Group, and these results compared with C Group,they hadno significantly differences. These results indicated that tea polyphenols could rivalrythe damage of free radical to myocardial tissues, protect the activity of ATPase, andmaintain calcium homeostasis. Conclusion:(1) Tea polyphenols could significantly rivalry the increasement of the free radicaland the lipid peroxidation in acute exhausted rats' myocardial tissue,and can protect theinjury of the myocardium.(2) Tea polyphenols could remarkably suppress the decrease of activity ofNa⁺-K⁺-ATPase and Ca²⁺-ATPase in acute exhausted rats' myocardial tissue, accordinglyimprove the cardiac muscle energize, and promote the synthesis and utilization of ATP;(3) Tea polyphenols could resistant the calcium overloading in acute exhausted ratsmyocardial tissues, thus block a series of harmful metabolism which evoked by thecalcium overloading.(4) Tea polyphenol could significantly extend the time of acute exhaustedswimming, elevate the ability of rats' movement, thus could have an effect on theresistance of sports fatigue;and its mechanism maybe that tea polyphenols can resist thethe calcium overloading in acute exhausted rats' myocardial tissue.