Studies Of Abalone Viscera Oil Extracted By Pulsed Electric Field(PEF), Its Refining And Antioxidation

With the development of Abalone and other shellfish industry, how to make useof the by-products and reduce the pollution becomes badly in need of resolving. Theby-products contain oil, protein, polysaccharide, microelement and vitamin, and thosebioactive substances can be fully utilized which will make economic benefits andreduce environmental pollution.This paper explored a new oil extraction technology from seafood--extraction ofabalone viscera oil with PEF.Firstly, the process parameters of PEF on oil extraction were studied. The yieldand disintegration index (Zp) were analyzed, then the best scope of each parameterwere determined by single factor analysis. The optimal technical parameters weredetermined by orthogonal analysis as follows: cement ratio was2:1, PEF strength was22.5kV/cm and processing time was40μs.Secondly, the PEF extraction was compared with Bligh-dyer method byanalyzing the physic-chemistry of the abalone viscera oil respectively. The resultshowed that the peroxide value, acid value and anisidine value in former were higherthan the later, and the iodine value was lower than later. The SFA was15.4%less,MUFA7.42%and PUFA7.98%more than later. The results showed that the PEF oilwas lower oxidation, higher degree of unsaturation and more excellent quality.Thirdly, the abalone viscera oil by PEF was refined. The processing parameterswere determined according to the physic-chemistry and the last yield. The result was:1.5%H3PO4(80%,v/v) in degumming,5%NaOH in neutralized,2%actived coal and1%actived earth as adsorbent in bleaching,60min/90℃in deodorization. Therefining steps were all carried out in a vacuum of approximately0.85MPa. Theprimary oil products were obtained, with acid value0.98and iodine value133.8.Finally, three different antioxidants (vitamin E, tea polyphenols and rosemary) were studied in this paper. The rosemary was proved to be the best antioxidant forabalone viscera oil, and the optimal addition was0.03%in the oil. Besides,0.2%VEwas effective in abalone viscera oil antioxidation. At last, complex naturalantioxidant "0.2%VE+0.02%rosemary" was proved to be the best effective inabalone viscera oil antioxidation.