The Effect Of Crude Polysaccharides In Abalone Viscus On The Physiological Function Of Diabetic Mice

In recent years,with people’s increasing need for abalone products and the development of artificial breeding and aquaculture technology,the abalone aquaculture has developed rapidly in the littoral of our country.China has become the world’s largest producer of abalone.Although the scale of aquaculture industry in our country has expanded quickly, the abalone products in the domestic market are mainly based on primary processed muscles of abalone.However,lots of abalone viscera which account for 25% of the total weight have been abandoned,and only a small number of viscera are processed into fodder.Some relevant studies showed that abalone viscera have high value in nutritional and medicinal properties.In our study,abalone viscera were used as raw materials to extract polysaccharides by ultrasonic technology and the optimum extracting condition was explored.The model of diabetic mice has been established to study the effect of crude polysaccharides on mice’s physiological function.The main researches and results were as follows:1.To select the best extraction technology of crude polysaccharides in abalone viscera:(1) Crude polysaccharides was extracted from abalone viscera by hot water.With extraction content as index,the extraction condition was optimized by single-factor experiments and orthogonal designs.During the extracting process,three main factors including extracting time.temperature and ratio of solid to liquid played important roles.Theywere studied separately.The best extraction conditions of crude polysaccharides were as follows:extraction temperature 50 ℃.extraction time 1.5h,ratio of material to liquid 1:8.(2) Ultrasonic treatment on the abstraction of abalone viscera crude polysaccharides was investigated.The extraction content was evaluated as markers for thestudy.To optimize the extraction condition,single-factor experiments and orthogonal designs were made.As extracting time,temperature,ratio of solid to liquid and ultrasonic power were the crucial factors in the process of extracting,they were studied to discover the best parameters.The best extraction conditions of crude polysaccharides were as follows:extraction temperature 60 ℃.extraction time 45 minute,ratio of material to liquid 1:8,ultrasonic power 160W.2.To investigate the effect of abalone viscera polysaccharide on the physiological function of diabetic rats which were induced by alloxan.(1) The effects of abalone viscera polysaccharide on the glucose tolerance and serum glucose level in diabetic mice were studied.The results indicated that abalone viscera crude polysaccharides has obvious effects on lowering the blood glucose in diabetic mice and improving impaired glucose tolerance in a certain extent.Meanwhile,it also played an apparent role on improving the life quality of diabetic mice and effectively relieving the typical symptoms of diabetes.(2) The effects of abalone viscera polysaccharide on the antioxidant ability in diabetic mice and its hypoglycemic mechanism were explored.The results showed that abalone viscera polysaccharide has obvious promoted the activity of SOD,GSH-Px and reduced the content of MDA in blood serum.The possible hypoglycemic mechanism was that abalone viscera polysaccharide could strengthen the activities of antioxidases and promote reparation and regeneration,of the cells which were damaged by injection of alloxan by eliminating redundant free radicals.(3) Abalone viscera polysaccharide’s protective effects on the organs of diabetic mice were investigated. The results proved that abalone viscera polysaccharide could certainly protected livers,kidneys and spleens of sick mice.it also improvd immune systems and minimized the seriousness of diabetic complications.The possible mechanism of its hypoglycemic activity was that abalone viscera polysaccharide could improve hepatic glucose metabolism by protecting the liver function.