| Mycotoxins are toxic chemical products formed as secondary metabolites by a few fungal species that readily colonise crops and contaminate them with toxins in the field or harvest. Aflatoxins B1is mycotoxins of major significance and hence there has been significant research on broad range of analytical and detection techniques that would be useful and practical. Not only the economic loss, but also the health risks for humans and animals cause the necessity to avoid or at least reduce the mycotoxin load. Fugal growth and mycotoxin formation cannot be avoided, therefore many method were developed to decontaminate feedingstuffs, to detoxify mycotoxins or reduce the bioavailability of mycotoxins.The content of this experiments was making a compound adsorbent MY, which was mixed Modified Bentonite and Yeast extract via a certain proportion. Then, compound adsorbent MY was studied the effect of it on the adsorpition of AFB1by adsorption experiment in vitro, evaluated the detoxification effect on mycotoxins by both poultry and livestock raise test and physiological biochemistry analysis in vivo.In vitro experiment:through screening of the yeast cell extract, bentonite, modified bentonite and different ratio of composite under the same conditions on AFB1adsorption effect, get on AFBi adsorption effect best modified bentonite:yeast cell extract complex (ratio3:7), the adsorption rate of89.40%, then through the study of different pH and adsorption stability, the results show that:the composite adsorbent can reached the maximum adsorption capacity in1hour, up to, the average adsorption rate, and lagest adsorption amount up to91.97%was at pH=7.0, Along with the pH reduction, adsorption rate decresed. Adsorption of methanol on complex stability influence was not so significantly.In vivo experiment (a)Experiments were conducted to evaluate the efficacy of MY to protect against aflatoxicosis of broliers. A total of one hundred and twenty1-day-old chickens were randomLy assigned to four treatment groups (three replicates per treatment with ten chick per replicate). Four dietary treatments of control feed, control feed plus113μg/kg AFB1, control feed plus113μg/kg AFB1and0.5%MY, control feed plus113μg/kg AFB1and0.5%MY were fed to treatment groups for28 days. The results showed that1) AFB1can significantly affect the content of Serum total protein and albumin;2) The addition of0.5%MY to AFB1-contaminated diet can effectively diminish the adverse effects of aflatoxinB1on hepatic function of chickens.In vivo experiment (b)Experiments were conducted to evaluate the efficacy of MY to protect against aflatoxicosis of pigs. A total of ninety77-day-old Da-Chang-Bai were identified by individual ear tags and tat-toos, and randomly assigned to three treatment groups (three replicates per treatment with ten pigs per replicate). Three dietary treatments of control feed, control feed plus92.17μg/kg AFB1, control feed plus92.17μg/kg AFB1and0.5%MY were fed to treatment groups for90days. Feed and water were provided for adlibitum consumption. The term of experiment is ninety days. The results showed that:1) AFB1can significantly affect pig health and growth performance, decrease body weight gain and feed conversion ratio, injure hepatic function, then cause significant alterations of related parameters;2) the addition of0.5%MY to AFB1-contaminated diet can effectively diminish the adverse effects of aflatoxin Blon growth performance and hepatic function of pigs.In conclusion, product MY could bind AFB1effectively in vitro and in vivo. These results indicated that product MY could alleviate some of the toxic effect in broilers and finishing pigs. It might prove be beneficicial in the management of afltoxin-contaminated feedstuffs for poultry and livestock. |
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