The Research Of Coptis Decoction Ejiao Revision Formulation Optimization And Effect Mechanism For Treat Chronic Nonbacterial Prostatitis

Objective:This topic based on the Classical Prescriptions which is Coptis Decoction Ejiao experience side to treating Chronic Nonbacterial Prostatitis(CNP) for obtain good results in clinical.We used the CNP Model by observing rats on serum TNF-α, the expression of iNOS, pathological changesand transmission electron microscopy(TEM) of impact, further verify the efficacy of treat CNP to explore its mechanism of action, and optimization the Coptis Decoction Ejiao Adjust for CNP clinical treatment to provide experimental basis that further lay the foundation for the development of new drugs.Methods:We take the 3 month old male wistar Rats with 72, were randomly divided into 2 groups: In model' using Zhang Ya-qiang rat prostate tissue antigen induced model of autoimmune prostatitis modeling with 60 Rats; 12 rats the saline group were anaesthetized with ether,12 rats the saline group were anaesthetized with ether and intraperitoneal injection of normal saline given more intradermal injections.45 days later, from the modeling with the saline group in each were respectively remove the 12 Rats out, orbital blood samples to detect TNF-a, to determine the modeling success or not.After successful, let the saline group 12 Rats only as a control groupⅠ; 60 Rats the model group were randomly divided into 5 groups:Pathological model groupⅡwere 12 in, Qianlietai groupⅢof 12 rats, Coptis Decoction Ejiao experience side group, granule group, capsule group were be groupⅣ,Ⅴ,Ⅵ, each group were 12 rats, and give it appropriate medication.After 4 weeks treatment, observed the rats in each group of general situation, Serum TNF-a levels, conventional light microscopy of prostate pathological changes, used the Immunohistochemical method to detection of prostate tissue expression levels of iNOS' and use TEM to observe prostate tissue of the Ultrastructure.Results:Compared with the control group, group of pathological model of prostate wet weight, wet weight/body weight ratio increased, Elevated serum levels of TNF-a, INOS expression in prostate tissue increased, significant difference (P<0.01), light microscopy, electron microscopy Showing the pathological of prostate tissue inflammation was changed, suggesting that the experimental model reproduced successfully.The three formulations of Coptis Decoction Ejiao experience side, it was reduced the average wet weight of prostate tissue, prostate wet weight/body weight ratio, and compared with the pathological model (P<0.01, P<0.05), Decoctions has better effect.The three formulations of Coptis Decoction Ejiao experience side, it was reduced the levels of serum TNF-a, and compared with the pathological model (P<0.01), Decoctions has good effect; It was reduced INOS expression in prostate tissue, Decoctions significantly.Light microscopy, electron microscopy showing the three formulations of Coptis Decoction Ejiao experience side were improved in the role of inflammation in prostate tissue, Decoctions is superior.Conclusion:The experiment found that:(1) The Decoctions, Granules, Capsules of Coptis Decoction Ejiao experience side can be effective treatment of CNP, the results showed that CNP has a Yin deficiency of Kidney, Firelight Kang, damp heat, blood stasis in the pathogenesis for clinical treatment of CNP, that provides a new way of thinking.(2) The three formulations of Coptis Decoction Ejiao experience side Probably by reduced levels of serum TNF-a, inhibit the expression of iNOS and improve other aspects of prostate pathology inhibit inflammatory damage, and valid.(3) The Decoctions of Coptis Decoction Ejiao experience side whole more effective than granules, capsules, the results indicate the Decoctions effect was the best, that provides a new way of thinking, for Classical Prescriptions Dosage forms Optimal in clinical.