Expression Of DNA Fragmentation Factor 45 And The Relationship With HPV Infection In Cervical Cancer

ObjectiveTo explore the expression and its significance of DNA fragmentation factor in normal cervical tissues, cervical intraepithelial neoplasms and cervical cancer. To analysis the relationship between the expression of DFF45 and the infection of HPV. To provide a scientific basic for exploring a new target of gene therapy.Methods(1) Immunohistochemical method(PV method)was used to detect the expression of DFF45 in in normal cervical tissues, cervical intraepithelial neoplasms and cervical cancer. Among these 48 cases were CIN (12 cases were CIN I and 36 cases were CIN II-Ⅲ); 41 cases were cervical cancer, of which 30 cases of squamous cell carcinoma (they were graded according to histology degree: well-differentiated cervical cancer, 10 samples; moderate differentiated cervical cancer, 12 samples; and 8 samples were poorly differentiated), adenocarcinoma 11 patients; Clinical stage according the criterion of FIGO 2009, Stage I, 23 samples, Stage II, 18 samples; With regional lymph node invasion group(10 cases)and without regional lymph node invasion group(31 cases). (2) Among 41 cases of CC, 28 cases were taken vaginal discharge before operation, gene chip method was used to detect HPV, then to analysis the relationship between the expressin of DFF45 protein and the infection of HPV.Results(1) Immunohistochemical results show that DFF45 protein was located in the cytoplasm and nucli. Which was distributed meanly and granulaly. (2) The positive expression rate of DFF45 protein in cervical cancer was showed higher than that in normal cervical tissues and cervical intraepithelial neoplasms, Which is significantly different (P<0.05). (3) No statistical difference was found between the tissues of CIN I and normal cervical tissues of DFF45 protein(P>0.05). The expression of DFF45 protein in CIN II-III was higher than that in CIN I, which was significantly different(P<0.05). (4) The expression of DFF45 protein was related with clinical stage and HPV infection (P<0.05). There is no relationship of age, histological type, histological degree and lymph node metastasis(P>0.05).Conclusions(1) No statistical difference was found between the tissues of normal cervical tissues and CIN I. DFF45 protein had no function in the early stage of the cervical lesions. (2) The expressin of DFF45 protein increased from CIN I, CINII-III to CC, from stage I to stage II. which was significantly different. DFF45 played an important role in the process which develops from High-grade cervical intraepithelial neoplasms to cervical cancer. (3) The expression of DFF45 protein was closely related with the infection of HPV. DFF45 and HPV coordinated and promoted each other in the occurrence and progression of cervical cancer. (4) It was supposed that the high expression of DFF45 protein was related with the inhibition of apoptosis, which is expected to be a gene therapy target of cancer.