Studies On The Technology Of Supercritical CO₂and Homognate Extraction Of Active Components From Sophora Japonic A And Peanuts Waste Meterial

Sophora japonica L. and peanuts are rich natural resources in China. Polyphenols of peanut testa andflavonoids of Sophora japonica and peanut shells with a variety of biological activity are widely used inthe fields of food, medicines, cosmetics and health products. In this paper, we take the Sophora japonicaand waste meterial of peanut in Kaifeng outskirts as research subject. The optimum technology ofextracting total flavones of Sophora japonica.and peanut shell by supercritical CO₂was established.Moreover high-speed countercurrent chromatography was developed for separating two flavonoids fromSophora japonica. And the technology of homogenate extraction was developed to extract the totalpolyphenol of peanut testa. The main contents are as follows:1. Review on the development of Sophora japonica. and peanut waste materialA brief overview of Sophora japonica and peanut waste meterial in recently years is reviewed,inclcding extraction, separation, purification, determination and physiological activity, and theshortcomings of traditional extraction process are summarized. On these basises, we put forward thesubject–to create a new "green" and efficient extraction process.2. Study on technology of supercritical CO₂extraction of total flavones from Sophora japonicaand peanut shellAdding the sodium dodecyl benzene sulfonate （SDBS）, the extraction rate of total flavones fromSophora japonica increased by0.937times. The optimal technology parameters: temperature50℃,pressure350bar, SDBS amount2%,75%ethanol concentration. Under these conditions, the averageextraction rate of total flavones was32.16%. The most suitable surfactant to extract total flavones from peanut shells by supercritical CO2wasammonium lauryl sulfate. The addition of ammonium lauryl sulfate enhanced Peanut shells flavonoids’extraction to28.39%, which was0.822times than that without added surfactant.3. Application of ionic liquids in separation and preparation of the two flavonoids of Sophorajaponica by HSCCCTwo flavonoids were prepared, isolated and purified from Sophora japonica by high-speedcountercurrent chromatography （HSCCC） using ionic liquids to improve the separation effect. A two-phasesolvent system composed of n-hexane-ethylacetate-ethanol-water-acetic acid （1:1:1:1:0.05, v/v） was used.The results indicated that18.2mg of rutin and9.6mg of quercetin with their purities over97%wereobtained from50mg crude extract of Sophora japonica. The peak time was advanced from85min to55min by adding Ionic liquids, the degree of separation from0.9to1.8, which had achieved completeseparation. The separation was significantly improved. The established method can be used for theseparation and purification of flavonoids from the Sophora japonica.4. Homogenate extraction and purification of the total polyphenol from peanut red flashThe optimum technology of extracting total Polyphenols of peanut testa by homogenate extractionwas established using single factor experiment and orthogonal test. The optimum extraction conditionswere as follows: ethanol concentration of80%, solid to liquid ratio of1:25（g/mL）,90V power, two timesextraction, each for2min. Under this conditions, extraction rate of total polyphenols was96.94%.Polyphenols were enriched and purifted by AB-8resin. The purity and yield of the purified polyphenolproduct was63.76%and12.3%, respectively.