Separation And Purification Of Bitespiramycin From Fermentation Broth By Resin

Bitespiramycin is a new macrolide antibiotic that has been developed by Chinese independently. It has complex structure and contains 10 kinds of 4"-acylated spiramycin derivatives among which isovalerylsiramycinsⅠ、ⅡandⅢhas been regard as the primary component.The adsorption method by resin was used to pretreat and separate the Bitespiramycin fermentation broth. And the process factors were studied and optimized. On account of the low amount of the target material, the research was focus on getting good quantity Bitespiramycin in a high yield. This paper includes the study of pretreatment/separation and optimization of the process factors, such as:Bitespiramycin fermentation broth was pretreated by inorganic membrane and resins. When the resin D293 was used as adsorbent to handle 12BV Bitespiramycin fermentation broth,73%of broth and 21%of protein can be removed while the loss of target material was only 2.6%. The impurity washing was added between absorption and elution unit. The washing medium was chosen first and the suitable concentration of washing buffer was also investigated. The petroleum eluent from the elute process was salified and the product obtained can reach quality standard, at the same time the yield of BitespiramycinⅢwas improved 85%or so.