The Research And Realization Of The Open Three-dimensional Web HPCE Virtual Laboratory

As a new hi-tech, virtual reality technology has been widely used in various fields. In the field of education, virtual laboratories promote the rapid development of new technologies such as remote education and online multiplayer collaboration. High performance capillary electrophoresis is one of the most dynamic field of cutting-edge researches in contemporary Analytical Chemistry. The problems are that complex structure of the instrument, time-consuming experimental pretreatment, difficult instrument routine maintenance, capillary electrophoresis peaks process is affected by a variety of factors such as voltage, pH and so on, the mathematical theory model is very complex. So, building a capillary electrophoresis virtual laboratory has significant meaning and value. However, the build process involves theoretical modeling, process design and optimization, algorithm conversion between theoretical model and programs and experimental knowledge of operating experience, which puts forward higher requirements of comprehensive capabilities for developers. What’s more, studies combined workstations spectrum simulation and high immersive, highly interactive3D virtual laboratories which contains specific experimental process have not been reported. Based on the reasons above, the software is researched and developed.Since capillary electrophoresis theoretical model is affected by many micro factors, such as the capillary wall ZeTa potential, charge density and so on. The theoretical model coefficients are difficult to determine. It is hard to simulate real-time dynamic capillary electrophoresis peaks according to the existing basis of theoretical model in a virtual laboratory. So based on the existing capillary zone electrophoresis theory, this paper extracted the relevant affecting factors such as temperature, voltage, pH, inlet sample pressure as well as injection time, through the integration and optimization, completed the theoretical models forecast, including peak time model of capillary electrophoresis, the relationship model of half peak width and peak time, and the relationship model between injection pressure, injection time and peak area. Then wrote simulate the peak program through the Algorithm conversion between theoretical model and programs. On this basis, developed a multifunctional capillary electrophoresis workstation, realized two-dimensional chromatographic data dynamic simulation and the functions of chromatographic data collection, storage and analysis of processing under different parameter conditions. Compared the simulated and real results which turns out that in a certain range of parameters, the error between virtual and real results is less than8%, the proposed model can reflect the influence rule of various parameters conditions on the peaks of capillary electrophoresis, which proved the rationality of the theoretical model constructed.In addition, the paper built capillary electrophoresis virtual lablaboratories combined with high immersive scenes, real-time interactive operation intervention and interference and spectra simulation workstations by using the software of3ds MAX, Virtools and so on. Realized six function modules which are the theoretical study, familiar instrument structure, various pretreatments, qualitative analysis, quantitative analysis and instrument maintenance and repair. Also achieved other auxiliary functions such as real-time tracking and evaluation Finally, successfully added the capillary electrophoresis virtual laboratory to the Dalian University of Technology experimental information management platform developed based on the Apache+PHP+MySQL technologies to achieving networking. The constructed virtual labs have powerful function, realistic scenes, high interactive interaction, which provides a good instrument for experimental teaching. Open system architecture not only can improve software network speed, but also help to expand the project easily. Its design philosophy and development ideas have a guiding role for building other instrumental analysis virtual laboratory simulation software.