Research On Anthocyanin Analysis Techniques And Anthocyanins Inheritance Of Parentoffspring Generations

Column chromatography was used to isolated anthocyanin form C. Chekiangoleosa, C. semiserrata, C. hiemalis’ShiShi Gashira’, C. azalea and C. reticulata, with packing of MCI gel CHP-20p and C18.7types of anthocyanins were isolated, confirmed as Cy3G, Cy3Ga, Cy3GX, Cy3GaX, Cy3GEpC, Cy3GaEpC and Cy3GEpCX by detection of HPLC and ESI-MS. All of the anthocyanins with purity over80%and even over95%in Cy3G,Cy3Gx and Cy3GEpC,which can be used as standard samples of HPLC.Anthocyanins of3pairs of parentoffspring generations were detected by High Performance Liquid Chromatography(HPLC) with UV/Vis Detector, and confirmed as Cy3Ga, Cy3G, Cy3GaZpC, Cy3GECaf, Cy3GZpC, Cy3GaEpC and Cy3GEpC. Quantitative analysis results show that the Cy3G and Cy3GX holds the largest proportion of total anthocyanins, which conform to the typical characteristics of C. Japonica. the RHS results indicated that high proportion of cy3g was highly correlated to crimson; anthoyanins with organic acid groups were correlated to purple red. The results of inheritance analysis of anthocyanins between parent offspring generations show that inheritance mode of Cy3G,Cy3Ga and Cy3GEpC tend to paternal. Therefore, it seems that paternal inheritance is common between C. Japonica hybridization.27azalea samples of seven pairs of azalea parents and their hybrids were analyzed by HPLC with UV/Vis Detector, and15major anthocyanins were detected. All the anthocyanins were classified into two groups of Cy series and Dp series. The results of quantitative analysis showed that Cy3Ga, Cy3G, pigment2, pigment5and pigment14as the main anthocyanins in azalea petals, with accumulated content over90%of total anthocyanins, and Dp series was only detected in4samples. Inheritance analysis indicated that Cy series of anthocyanins was dominantly controlled by paternal inheritance, while Dp series probably by recessive inheritance. The results also showed that inheritance pattern of Cy3G5G, pigment15are dominated by enhancer genes of multiple genes; pigment5, pigment14and Cy3G are controlled by equivalent genes; pigment2, pigment4and Cy3G are dominated by reductive genes. Therefore we deduce that flavonoid3-O-glucosyltransferase and flavonoid3-O-galactosyltransferase are respectively controlled by reductive genes and equivalent genes; flavonoid5-O-galactosyltransferase is controlled by enhancer genes. The color results showed that Dp series anthocyanins are highly correlated with blue-purple in azalea flowers; pigment14of Cy series anthocyanins is correlated with magenta flowers, and other anthocyanins of Cy series are correlated with scarlet flowers. Strategies for color breeding with anthocyanin assistantance were brought forward.