Monospore Hybridization And Selecting Of Progenies In Hericium Erinaceus

In this experiment,the parent strains of Hericium erinaceus were H.erinaceus No1 and hericiumcaput- medusae. 143 new progenies strains were obtained by monospore hybridization,and those strains were used to attain hericium erinaceus strains with properties of good quality by a series of selecting experiments.1.118 new hybridization progenies strains were obtained by microscope checking, antagonistic and fruiting experiments.2.21 new filial generation with strong heterosis were obtained by means of vertical slab polyacrylamid gel electrophoresis and analysis in esterase isozyme of crossing parents and new cross combination, which had mutually complementary enzyme bands and new enzyme bands.3.Compared about the mycelium growth, H2,H9,H21,H38 and H94 are excellent; The experiment results showed significant positive correlation between average growth rate of mother cultures and average growth rate of culture spawn. The experiment results also showed correlation between average growth rate of culture spawn and biotical efficient, but the correlation is not significant. Five strains as H9,H16,H29,H94 and H100 had the ability of high temperature tolerance.4.The result showed that fruit body yields of four strains as H16 ,H48,H94 and H100 were higher than their parental strains, four strains as H2,H21,H29 and H76 were higher than one of their parental strains, it was proved that the heterosis of these new crossing progenies strains in fruit body yield was strong. Four strains as H9,H21,H53 and H94 were early-mature strains because of their earlier peak fruiting periods. The experiment results also showed that some correlation between peak fruiting period and biotical efficient, but the correlation was not significant.5.Through comparing the resistance to Trichoderma Viride and characteristics of fruiting bodies, five strains as H9,H16,H21,H38 and H94 had stronger resistance to Trichoderma Viride, among which H38 was the strongest. H53,H76,H100 and H103 strains were much better than others in characteristics , which showed bigger fruiting body, compact fruitbody ,good fruitbody shape and high output of per fruitbody. 6.Tow excellent strain resources were obtained.H16 resist to Trichoderma Viride, and has high output.H100 has high temperature tolerance,and high output.