Studies On Requirement Levels Of Energy In Wheat-based Meal Diets Adding Non-starch Polysaccharides Enzyme For Laying Hens

This experiment was conducted to study effects of different levels of energy on production performance, egg quality, nutrients utilization, expression of Glucose transport carrier of hens under the conditions of adding non-starch polysaccharides (NSP) enzyme to wheat meal diets.Using single factors experiment design, select 1024 new Isa Brown hens 210 days of age, which is divided into 8 groups, each group has 4 repeats. Group 1 was the control group ,fed corn diet ; group 2 was the negative control group , fed wheat diet. Group 3 was test group ,fed wheat add 0.01% enzymes diet . Other groups using zeolite instead of vegetable oil with powder amount to gradually reduce energy 0.2MJ/kg, which composed of 8 kinds of diets. Back-grounding 7d, trial period 90d, Results indicate:①The egg rate than the control group and group are no significant difference(P>0.05).Than the negative control group were higher: 8.00%、7.04%、6.72%、6.71%、5.91%、3.81%.That are significantly different(P<0.05). intake of group 3, 4, 5, and 6 than group 7, 8 were low 9.80%、6.72%、5.83%;7.26%、5.53%、4.08%;5.43%、3.66%、2.19%;3.81%、2.01%、0.51%,and that was significant. Egg weight have to decline. F/E of group 7、8 lower than other groups(P<0.05).②The amount of cholesterol in egg of group 8 was significantly lower than other groups(P<0.05),it than the other group low : 1.86%、0.39%、2.6%、2.29%、1.98%、1.67%、0.26%. The yolk color and hastelloy units in group 1 and group 2 ,but there were no significant different among other experimental groups(P>0.05).Group 2 was significantly lower than other group(sP<0.05),low : 4.9%、5.37%、3.96%、4.9%、2.51%、1.52%、1.52%. Group 3 was significantly lower than other groups(P<0.05),low: 5.46%、2.38%、0.212%、0.458%、1.53%、2.52%、4.04%.Group 6 was significantly higher than other groups(P<0.05),high: 4.22%、9.54%、3.15%、3.66%、5.61%、6.06%、8.89%.③Group 1 was significantly lower than other groups(P<0.05),low: 16.6%、15.1%、16.5、15.8%、18.2%、19.7%、20.8%,that was significant difference. The digestibility of crude protein of group 2 were significantly lower than other group(sP<0.05),low: 40.6%、32.2%、33.4%、38.3%、40.6%、36.1%、38.0%. Group 1 was significantly lower than other groups(P<0.05),low: 6.24%、2.98%、2.63%、2.96%、4.77%、4.69%、5.99%.There were no significantly different than experimental groups with group 1.④That was no significant difference, The glucose content levels of mesenteric blood serum of each group. Group 2 was significantly lower than other groups(P<0.05),low: 3.95%、3.95%、3.41%、3.45%、3.01%、3.93%、3.88%. ⑤SGLT1 and GLUT of group 1 than the groups 2 of high, 39.7% and 20.5%. SGLT1 and GLUT of each group was no significant difference.