Study On Seed Propagation And Tissue Culture Of Rhododendron

Rhododendron which is very popular in the market in recent years has strong appreciation.It is China’s famous flowers and advantage resources.It is the characteristic plant in Shandong province, too. The seeds of Rh.simsii Planch.were used as our experimental material for seed germination experiment and the plant leaves, bacteria-free seedlings leaves, bacteria-free seedlings stem section were used for tissue culture.The main conclusions are as follows:1. The seeds were not processed with GA3in CK. Then the seeds were treated with20mg/L,50mg/L,100mg/L,200mg/L GA3and processing time were6h,12h,24h,36h respectively under four concentration condition. Compared with the CK, GA3promoted the seed germination.100mg/L GA3show the best effct on the seeds germination.2. Two methods were used for germination experiment. One method was that germination experiment was done in petri dishes for9centimeters in which filter paper wre put. The second method was that the experiment were done on1/4Anderson culture medium. The condition of seed germination experiment was that petri dishes were put into light incubator under the25℃condition for12hours of light which was2000lux approximately in intensity.The experimental result showed that seeds of R.simsii Planch.needed light.3. Anderson medium was the basic medium. Shoot differentiation induction experiment was done on Anderson culture medium,1/2Anderson culture medium,1/4Anderson culture medium,1/8Anderson culture medium in which joined additional different hormone.1/4Anderson culture medium was determined the optimum medium in the last.4. The plant leaves, bacteria-free seedlings leaves, bacteria-free seedlings stem section were used as explants. The experimental result showed that when plant leaves were used as explants, the optimum medium was1/4Anderson+1.0mg/L2,4-D+0.5mg/L6-BA and the rate of callus formation was up to92%. When bacteria-free seedlings leaves were used, the optimum medium was1/4Anderson+1.0mg/L2,4-D+0.5mg/L6-BA.The rate of callus formation was up to100%on this medium. When bacteria-free seedlings stem section were used, the optimum medium was1/4Anderson+1.0mg/L2,4-D+0.5mg/L6-BA and the rate of callus formation was up to95.3%5. When plant leaves were put on shoot differentiation medium, the explants didn’t show the bud differentiation. When the bacteria-free seedlings leaves were used, the explants showed the bud differentiation occasionally. The rate of shoot differentiation significantly increased when callus of plant leaves and bacteria-free seedlings leaves were used. The rate of shoot differentiation was up to45.0%and48.3%separately. The result showed that callus of bacteria-free seedlings leaves was more suitable for bud induction.The medium was1/4Anderson+0.1mg/L IBA+1.0mg/L TDZ.6. In seedling cultivation experiment,1/4Anderson was used as basic medium.When0.1mg/LNAA and1.0mg/L ZT were put onto the1/4Anderson medium, the seedlings growed strongly. It was suitable for the next root training test.7. In rooting experiment,1/4Anderson was used as basic medium. When2.0mg/L IAA and0.1mg/L NAA were put into the1/4Anderson medium, it was most conductive to seedling rooting.8. The vermiculite and peaty soil were used in tried seedlings experiment and the proportion was2:1.