The Effects Of Chinese Herbs On Physiobiochemical Parameters And Disease Resistance Of Epinephelus Malabaricus

In order to study the effect of the Chinese herbal medicine on the physiobiochemicalparameters and antiviral ability of E. malabaricus, different level of Chinese herbal medicinewere added in diet. According to the results, one of appropriate Chinese herbal medicine andits appropriate level were selected. Another possible way coulde be put forward to solveproblem such as residues of fishery drugs, reduce diseases in aquaculture, and enrich thefundamental researches of Chinese herbal medicine used as promoting its green and healthydevelopment, while make Chinese herbal medicine compound formula become scientific.1Simple prescription of Chinese herbal medicine: one thousand and two hundredhealthy E. malabaricus whose body weight was (83.08±0.58) g were randomly divided into10groups with3replicates and40groupers in each replicate in the first trail. lonicerajaponica Thunb, Phellodendron amurense Rupr and Gardenia jasm inoides Ellis were addedin the basic diet by5,10and20g/kg. Physiobiochemical parameters of E. malabaricu weretested at7thday,14thday,28thday. The results showed that the addition of5g/kg of lonicerajaponica Thunb in the diet has the best effect on the lysozyme (LZM) activity of serum andantibacterial of skin mucus. When the addition was10g/kg of lonicera japonica Thunb, LZMactivity of skin mucus, nitric oxide (NO) content, albumin (ALB) content, phagocyticpercentage (PP), malondiadehyde (MDA) content, albumin/globulin (A/G) ratio, uric acid(UA) content had the best effect. The addition of20g/kg lonicera japonica Thunb in the diethad the best effect on the NBT positive cell count, white blood cells (WBC), activities ofsuperoxide (SOD), activities of total antioxidant capacity (T-AOC), toal protein (TP) content,cholesterol (CHO) content. And the results indicated that the creatinine (CR) content reachedthe minium value by5g/kg Phellodendron amurense Rupr. When the addition was10g/kgPhellodendron amurense Rupr, there was the best effects on the increased activities ofcatalase (CAT), reduced activities of xanthine oxidase (XOD) and urea nitrogen (BUN)content. The addition of20g/kg Phellodendron amurense Rupr in the diet had the best effecton increased red blood cells (RBC). The triglyceride (TG) content reached the maxium valueby20g/kg Gardenia jasm inoides Ellis. However, hemoglobin (HB) content and PI had not significant difference compared with control group in all of the experiment groups.Edwardsiella tarda infected E. malabaricu after28days of feeding diet with Chinese herbalmedicine. The results showed that the mortality rate of all experiment groups is lower than thecontrol group. The highest relative percent survival reached90%when it was fed with20g/kg lonicera japonica Thunb.2Prescrpition compatibility of Chinese herbal medicine: eight hundred and fourtyhealthy E. malabaricu whose body weight was (87.93±0.37) g were randomly divided into10groups with3replicates and40groupers in each replicate in the first trail. Chinese herbalmedicine compound Ⅰand Ⅱwere added in the basic diet by5,10,20g/kg. Physiobiochemicalparameters of E. malabaricu were tested at7thday,14thday,28thday. The results showed thatthe addition of5g/kg Chinese herbal medicine compoundⅠin the diet had the best effect onreduced UA, CHO content and increased antibacterial of skin mucus. The addition of10g/kgChinese herbal medicine compoundⅠin the diet had the best effect on TP, TG, MDA contentand CAT activity. When the addition of20g/kg Chinese herbal medicine compoundⅠ, LZMactivity, NO content reached maxium value and activity XOD reached minium value. Theaddition of5g/kg Chinese herbal medicine compoundⅡin the diet had the best effect onreduced CR content and increased WBC and RBC. The addition of10g/kg Chinese herbalmedicine compoundⅡin the diet had the best effect on increased ALB content, PP, activities ofSOD, NBT positive cell count, activities of LZM of skin mucus and reduce A/G ratio. Theaddition of20g/kg Chinese herbal medicine compoundⅡin the diet had the best effect onreduced BUN content, activities of XOD and increased activities of T-AOC. However, HBcontent, WBC and PI had not significantly difference compared with control group in all ofthe experiment groups. E. tarda infected E. malabaricu after28days of feeding diet withChinese herbal medicine. The results showed that the mortality rate of all experiment groupswas lower than the control group. The Chinese herbal medicine compoundⅡin relative percentsurvival was higher than Chinese herbal medicine compoundⅠ. The highest relative percentsurvival reached90%when the addition of20g/kg Chinese herbal medicine compoundⅡinthe diet.