Study On Rapid Propagation In Vitro Of Hosta Plantaginea Of4New Cultivars

In this study. Four new Hosta varieties:"Hosta,’Gloden Cadet’","Hosta,’Gloden Tiaia’"."Hosta,’Gloden Eagle’","Hosta,’Proadside’" for test materials.A detailed study of the primary cultures.subculture proliferation,rooting of rapid progation in vitro process. To provide technical support for the Hosta in the Practical production. In this text, by different parts of Hosta and different sterilization method, different combinations of plant growth regulators for effect of primary culture, proliferation culture, rooting culture of explants and transplantation groundmass on survival rate etc. with systematic analysis, and in industrial production as a precondition for different the cultivation time on plantlet height, field planting number, illumination type etc.analysis of physical factors, find the best plantlet growth state as factory production to establish good foundation. Results show that:1. The best material parts for Hosta in vitro rapid propagation of parts of Hosta is Bud, can be directly induced from adventitious buds, shorting the access time no vaccine. Explants sterilization proposal:75%Alcohol30s+0.1%mercuric chloride sterilizing12min.2. Hosta "Gloden Cadet","Gloden Tiaia" the adventitious buds induced from the optimum medium is MS+BA2.5mg/L+NAA0.2mg/L;H"Gloden Eagle" the best medium is MS+BA4.0mg/L+NAA0.2mg/L;"Proadside" the best medium is MS+BA3.5mg/L+NAA0.2mg/L.3. Hosta "Gloden Cadet" of best proliferation medium was MS+BA2.0mg/L+NAA0.2mg/L,"Gloden Tiaia" of best proliferation medium was MS+BA2.0mg/L+NAA0.5mg/L,"Gloden Eagle" of best proliferation medium was MS+BA4.0mg/L+NAA0.2mg/L,"Proadside "of best proliferation medium was MS+BA3.0mg/L+NAA0.2mg/L. In the subculture, adventitious bud with bud amount to2-3best, subculture period of30d, shall not exceed5times of subculture.4. Hosta "Gloden Cadet" optimum rooting medium is MS+NAA0.5mg/L;"Gloden Tiaia" optimum rooting medium is MS+NAA0.3mg/L;"Proadside "optimum rooting medium is MS+NAA0.2mg/L;"Gloden Eagle" optimum rooting medium is MS.In the rooting, Rooting planting seedlings number8Cong/bottle,2-3plants/Cong5. Four Hosta species for the best transplanting substrate is perlite:peat=1:3, four varieties can be without hardening seedling transplanting.6. Four Hosta varieties of factory seedling breeding standards:subculture proliferation culture is plantlets heigh2-2.5cm, bottle inoculated8Cong. The rooting is plantlets heigh3-4cm, bottle inoculated8Cong. The best out of the bottle for plantlets height5-6cm,3-4root, root length1.5cm above. Factory production by water instead of distilled water. Sugar instead of Sucrose. pH value for the5.5-6.0...