The Study Of Tissue Culture Propagation Techniques On The Four New Vari Eties Of Hosta Plantaginea

In this study, four new varieties of Hosta, Hosta.‘Big Daddy’,Hosta.‘Gloden Eagle’,Hosta.‘Gloden Cadet’ and Hosta hybrida.‘Halcyon’ shoot tip as explants, in industrialized breeding as the groom goal, choosing MS medium as base medium, adding different hormone(6-BA、NAA、IBA、2,4-D)induced respectively to find out the most suitable cultivation methods on primary culture, following the optimum culture cultured, callus induction, rooting and transplanting in the process of radical concentration aim to provide the basis for industrialized seedling.1. The best season drawn of Hosta explants of Hosta.‘Big Daddy’, Hosta.‘Gloden Eagle’,Hosta hybrida.‘Halcyon’were April,and the Hosta.‘Gloden Cadet’ were July;2. About the start of the training process, Hosta.‘Big Daddy’ and Hosta hybrida.‘Halcyon’ were both cultured in MS+4.0mg/L6-BA+0.2mg/LNAA medium with 100%primary initiation rate, while the explants of Hosta.‘Gloden Eagle’ and Hosta.‘Gloden Cadet’were 84.6% and 83.3% as the highest rate of initiation in culture medium of MS+3.5mg/L6-BA+0.2mg/LNAA;3. In callus induction, Hosta.‘Big Daddy’ showed the best effect induced by 4 times in MS+0.3mg/L6-BA+0.1mg/L2,4-D culture medium,Hosta.‘Gloden Eagle’ 、 Hosta.‘Gloden Cadet’ 、 Hosta hybrida.‘Halcyon’ are the most powerful in the culture medium of MS+0.3mg/L6-BA+0.01mg/L2,4-D, respectively the Hosta.‘Gloden Eagle’ is 3.2,and Hosta.‘Gloden Cadet’2.0,Hosta hybrida.‘Halcyon’1.2;4.In the 4 subculture, By 1.0mg/L6-BA+0.3mg/LNAA+35g/L sucrose +12g/L agar medium Hosta.‘Big Daddy’ and Hosta.‘Gloden Eagle’ can the maximum subculture added multiples of 2.54 and 2.78. Hosta.‘Gloden Cadet’ can be 2.91 by medium of 1.0 mg/L6-BA+0.2mg/LNAA+30g/L sucrose +11g/L agar.The Hosta hybrida.‘Halcyon’ can be 2.10 the medium 3.0mg/L6-BA+0.1mg/LNAA+30g/L sucrose +12g/L agar;5.When each generation plant with bud proliferation in 4-5, the four Hosta can beinductede to get the maximum proliferation multiples,Hosta.‘Gloden Cadet’ and Hosta.‘Gloden Eagle’ were 2.17 times, Hosta.‘Big Daddy’ was 1.5 times,and Hosta hybrida.‘Halcyon’ were1.33 times;6.Hosta.‘Big Daddy’ and Hosta.‘Gloden Cadet’ are 12 strains inoculation density bottle of showed the greatest proliferation rate, respectively 2.75 and 2.67, Hosta.‘Gloden Eagle’and Hosta hybrida.‘Halcyon’ in 10 strains inoculation density bottle are multiplication,respectively 2.00 and 1.40;7. In promoting root growth medium screening, MS + 0.05 mg/L 2,4-D + 1.0 mg/L NAA+2.0mg/LIBA+10g/L agar culture medium can induce four Hosta root elongation growth is most effective, four Hosta average root length were, the Hosta.‘Big Daddy’ is 0.88 cm,7.64 cm,5.65 cm and 1.53 cm, MS+0.05mg/L2,4-D+1.0mg/, LIBA+12g/L agar culture medium can be prompted four Hosta cultivars seedling height growth has the best effect, the average height of seedling were as high as the Hosta.‘Big Daddy’ is 5.28 cm, Hosta.‘Gloden Eagle’4.66 cm,Hosta.‘Gloden Cadet’ 2.78 cm and 2.05 cm while MS+0.1mg /L2,4-D +1.0mg/L NAA+ 0.5mg/L IBA+12g/L agar base number can be inducted rooting of tissue culture seedlings reached the large number of varieties, four average root, for the Hosta.‘Big Daddy’ is7,Hosta.‘Gloden Eagle’ is 13,4 and Hosta hybrida.‘Halcyon’ 2;8.Refined seedling transplanting in indoor 24-26 ℃, open the bottle caps, after exposure to place 2-3 d can be transplanting to culture in a matrix, choice of sand soils and pure sand substrate can be the optimal effect of promoting the somaclone survival.