Effect Of Arginineon The Intestinal Protein Synthesis,Expression Of Amino Acid Transport Protein MRNA An1Gut Health In The Fine-Wool Sheep

To test the ef fect of the different dietary levels of rumen-protected arginine (RP-Arg) and rumen-protected N-carbamylglutamic acid (RP-NCG) on the intestinal protein synthesis, expression of amino acid transport protein mRNA and gut health in the weaned Fine-Wool sheep.the trials were divided into four parts.Part1Assessment of the digestibility and Rumen fermentation function of basal diet in vitro gas production and fermentation test and two-stage in vitro digestibility testThis study determined the digestibility and Rumen fermentation function of basal diet. Three healthy cashmer Goats (BW～40kg) were selected as test animals supplying rumen liquor. In the morning, Collected the rumen liquor before feeding, and then started the in vitro gas production and fermentation test and two-stage in vitro digestibility test. The results indicated that the PH and the concentration of Ammonia Nitrogen were within the normal range, the DMD, DCP、DNDF and Gas Production of basal diet were higher.Part2The Study of Protein synthesis rate of intestinal mucosa for weaned Fine-Wool lambs in dietary supplementation with rumen-protected Arginine (RP-Arg) and rumen-protected N-carbamylglutamic acid (RP-NCG)This study determined effects of dietary supplementation with rumen-protected Arginine (RP-Arg) and rumen-protected N-carbamylglutamic acid (RP-NCG) on the fractional protein synthesis rates of intestinal mucosa in weaned Fine-Wool lambs, fifteen weaned Fine-Wool lambs (BW25±3.39kg),5-month-old, were randomly divided into five groups:Basal diet group(group one), Basal diet+RP-Arg-1.5g/d group(group two), Basal diet+RP-Arg-2.0g/d group(group three), Basal diet+RP-NCG-0.15g/d group(group four) and Basal diet+RP-NCG0.2g/d (group five). Following a-45d period of feeding, at12h after the last meal, the animals were injected a flooding-dose of L-[ring-D5] phenylalanine, and then the duodenal mucosa and jejunum mucosa samples were collected after90min postinjection to measure the fractional protein synthesis rates (FSR). The results showed that the FSR of duodenal mucosa and jejunum mucosa in all treatments were higher then the control group (P＜0.05). the RP-NCG-0.20, RP-Arg-2and RP-Arg-1.5enhanced the FSR heighly then the control, especially RP-Arg-1.5enhanced the FSR significantly then the other treaments(P＜0.05). Collectively, these results indicate that dietary RP-Arg and RP-NCG supplementation could increase the protein synthesis rate of intestinal mucosa. Part3The Study of the mRNA expression level of Basic amino acids transporter for weaned Fine-Wool lambs in dietary supplementation with rumen-protected Arginine (RP-Arg) and rumen-protected N-carbamylglutamic acid (RP-NCG)This study to test the effects of dietary supplementation with rumen-protected Arginine (RP-Arg) and rumen-protected N-carbamylglutamic acid (RP-NCG) on the mRNA expression level of of candidate AA transporter genes solute carrier (SLC) family3, member1(SLC3A1), SLC7A9, SLC3A2and SLC7A1encoding for rBAT, b0’＋AT,4F2hc and CAT-1, respectively, in the jejunum and duodenum. The laboratory animals and the groups of exprement were the same as the exprement2. At the end of a45-day feeding period, Samples of intestinal muscosa were collected for the assessment of abundance of above candidate AA transporter genes. The result indicated that dietary supplementation with rumen-protected Arginine (RP-Arg) and rumen-protected N-carbamylglutamic acid (RP-NCG) could enhance the mRNA relative expression of SLC3A1, SLC7A9,SLC3A2and SLC7A1mRNA in duodenum and jejunum. In the jejunum, the mRNA relative expression of above genes which were significantly differences between the treatments and the control(P＜0.05). But in the duodenum there were not significantly differences among these groups (P＞0.05)Part4The Study of gut health for the weaned Fine-Wool sheep in dietary supplementation with rumen-protected Arginine (RP-Arg) and rumen-protected N-carbamylglutamic acid (RP-NCG)This study determined effects of dietary supplementation with rumen-protected Arginine (RP-Arg) and rumen-protected N-carbamylglutamic acid (RP-NCG) on the gut health in the weaned Fine-Wool sheep. The laboratory animals and the group of exprement were the same as the exprement2. The results indicate that:Concerning the gut immunity function, the results showed that the concentration of interleukin-2(IL-2), secretory immunoglobulin(SIgA) in the treatments were significantly higher than that in the control group (P＜0.05). Especially, the group4increased most significantly then the other treatments (P＜0.05).Collectively, these results suggest that dietary RP-Arg and RP-NCG supplementation could improve the gut immune function.Concerning the gut anti-oxydation ability, the results showed that dietary RP-Arg and RP-NCG supplementation could reduce the concentration of Malondialdehyde (MDA) and xanthine oxidase (XOD), and enhance the concentration of superoxide dismutase (TSOD) and the activity of glutathione peroxidase (GSH-Px). The3,4,5treatments were significantly higher than that in the control group (P＜0.05). Collectively, these results suggest that the arginine could ehance the gut anti-oxydation ability. Concerning the intestinal morphology, the results showed that using the light microscope, the villus height, crypt depth and their ratio were significantly higher then that in the control group (P＜0.05), All the treatments which were significantly differences in villus height and crypt depth(P＜0.05). The group4were significantly higher. Collectively, these results suggest that dietary RP-Arg and RP-NCG supplementation could protect the intestinal barrier function and improve the intestinal development.Concerning the intestinal Enzymes activity, the results showed that the alkaline phosphatase (AKP) concentration which is different significantly between the treatments and the cotrol (P＜0.05), and there were significantly differences among the treatments (P＜O.05). Collectively, these results suggest that dietary RP-Arg and RP-NCG supplementation could improve the gut digestive ability and promote the intestinal villus development.Concerning the and Nitric-Oxide Synthase (NOS), the results showed that dietary RP-Arg and RP-NCG supplementation could enhance the concentration of Nitric Oxide (NO), inducible nitric oxide synthas(iNOS) and TNOS. The3,4,5treatments were significantly higher than that in the control group (P＜0.05), Especially, the group4increased most significantly then the other treatments (P＜0.05) Collective ly, these results suggest that the arginine could ehance the anti-oxydation ability and probably by increasing the concentration of NO and NOS.From all results, we could detect that dietary supplementation with rumen-protected Arginine (RP-Arg) and rumen-protected N-carbamylglutamic acid (RP-NCG) could improve the gut health.