Effect Of Tea Saponin On Intestinal Coccidium, Blood Biochemical Index And Growth Performance Of Broilers

Tea saponin and tea seed meal were used in this study to investigate their effect on intestinal coccidium, growth performance, meat quality and blood biochemical index. Our results are as follows after analysis:(1) Effect of coccidiosisThe results indicated the tea saponin and tea seed meal could inhibit the sporulation of E.tellena oocysts.0.5%of tea saponin was the most effective on inhibition sporulation, followed by1.0%tea seed meal group. The sporulation rate of0.5%tea saponin group was39.38%, and decreased by38.53%on compared with the control group at36h. For oocysts reduced, the OPG in tea saponin groups all were decreased more than90%, and OPG in the tea seed meal groups were reduced more than80%. After the morphological structure, fluff length and other related index on the cecum epithelium of birds were analyzed, we thought that the tea saponin can be used in the diet as anticoccidial drugs, but the tea seed meal without detoxification could not be added in the diet.(2) Effect of growth performance of broilersComparison with the control, the average daily weight increased by8.74%(P<0.05), and the ratio of feed to weight decreased by15.52%(P<0.01), and the percentage of carcass weight increased by2.33%(P<0.05) when fed a basal diet supplemented with500mg/kg tea saponin. The ratio of feed to weight respectively decreased by5.60%(P<0.05) for300mg/kg tea saponin,10.34%(P<0.05) for500mg/kg tea saponin when compared the control.(3) Effect of meat quality of broilersComparison with the control, the dripping loss of breast and leg muscle in broilers was lower when different levels of tea saponin were added to the basal diet. The decrease of dripping loss in breast and leg respectively decreased by11.19%(P<0.01) and by13.51%(P<0.01) for400mg/kg tea saponin, by14.24%(P<0.01) and11.97%(P<0.01) respectively for500mg/kg tea saponin. The shear force of breast and leg respectively decreased by17.19%(P<0.05) and29.17%(P<0.05) when the addition of tea saponin was500mg/kg. Comparison with the control, the triglyceride (TG) in breast muscle decreased by27.42%(P<0.05) for400mg/kg tea saponin, and the triglyceride (TG) in leg muscle decreased by22.50%(P<0.05) for500mg/kg tea saponin. The difference of cholesterol (TC) level in breast muscle for each groups was not signigicant (P>0.05). The max value of TC decreased in leg flesh was9.13%(P<0.05) when the addition of tea saponin was400mg/kg. The difference of the pH value and water in muscle were not notable (P>0.05).(4) Effect of immune organs indexes of broilersWhen the addition of tea saponin was400mg/kg, the cloacal bursa index of broiler was significantly higher than that of control (P<0.05). There was no significant difference in thymus index and spleen index (P>0.05) when different levels of tea saponin were added to the basal diet, but showed an increasing trend. The results indicated that tea saponin can promote the development of animal immune organs, or improve the immune function of broilers.(5) Effect of biochemical index in bloodComparison with the control, the higher dose of tea saponin supplement could decrease TC and TG contents in serum. The TC level of serum decreased by30.72%(P<0.05) when the addition of tea saponin reached500mg/kg at day14. The most significant reduction ratio of TG contents in serum was40.15%(P<0.05) for400mg/kg tea saponin at day28. At day42, the best result for HDL-C in serum increased by19.08%(P<0.05) for400mg/kg tea saponin. TP, ALB, and GLO content in serum were improved, while LDL-C in serum was decreased, but the difference were also significant (P>0.05). The GLU level in serum increased by20.53%for500mg/kg tea saponin group at day14on compared with the control.