The Effect Of Environmental Factors On Spore Germination And Gametophyte Development Of Cibotium Barometz

Cibotium barometz is the member of the family Dicksoniaceae. It is a second class strate protection of wild species. The spore germination and gametophyte development of C. barometz is affected by environmental factors. In this study, we explored culture density, storage time, hormones (GA,6-BA, IAA), the gametophyte development of Sphenomeris chinensis, and the exogenous mature gametophyte of C. barometz impacted on the spore germination and gametophyte development of C. barometz by tissue culture, in order to provide a theoretical basis for the introduction, domestication, protection and cultivated breeding of C. barometz. The conclusions are as follows:(1) As the density of C. barometz increases, the spore germination rate showed decreased. In the early stage of culture, the difference between different density is not significant, it is significant in the late stage of culture. When the spore suspension of C. barometz is5μl, it’s spore germination rate is the highest. The gametophyte development of C. barometz is basically in the same speed, there was no significant difference between different density.(2)As spore storage time of C. barometz increases, it’s spore germination rate tended to increase, and the gametophyte length tended to decrease. Spore storage time for7days, the gametophyte length of C. barometz is the longest. When the storage time is630d, it’s spore germination rate is the highest, and the gametophyte length was significantly lower than it in the storage time of30d and7d.(3) With the increase of GA, the spore germination rate and gametophyte length of C. barometz increased first, then decreased. When the concentration of GA is0.02mg/L, the spore germination rate of C. barometz is the highest. When it is greater than or equal to0.03mg/L, it’s spore germination is inhibited. When it is0.05mg/L, the gametophyte development of C. barometz is inhibited. With the increase of6-BA, the spore germination rate of C. barometz increased first, then·decreased. When6-BA is0.04mg/L, it’s spore germination rate is the highest. With the increase of IAA, the spore germination and gametophyte length of C. barometz increased first, then decreased. When IAA is’0.02mg/L, it’s spore germination rate is the highest. When IAA is0.03mg/L, it’s gametophyte length is the highest. When IAA is greater than or equal to0.04mg/L, it’s spore germination is inhibited. When IAA is greater than or equal to0.05mg/L, it’s length of the gametophyte is inhibited.(4) With the increase of S. chinensis spore, the spore germination rate and gametophyte length of C. barometz is reduced.(5) With the exogenous mature gametophyte of C. barometz, the spore germination rate, gametophyte length, rhizoids length and the average number of rhizoids of C. barometz is higher than the control group.