The Effect Of Electro-acupuncture In The CytC Protein Expression Level Of Point Area

Objective:To study the electro-acupuncture point in the role of mitochondrial cytochrome c expression level through animal experiment, and to reveal the essence of Meridian laboratory of molecular biology to provide exact basis and provide clinical disease acupuncture practice with new ideas and methods.Method:Randomly divided30health level adult female SD mice into three groups: control group, anesthesia group and acupuncture group (and is divided into: non-points area group and itself on side Group), each group10only, control group and anesthesia group does not intervene, acupuncture group respectively give electric needle in right jiquan point and chongmen point, once a day, each20min, then do the operate at the same way, and after the experimental14th mice processing2hours, applied Immunohistochemistry technology and Western-blot technology to detect the expression of cytochrome c, different sides of observation on effect of acupuncture on expression of mitochondrial cytochrome c in point area.Result:Group1:the gray-scale value of the jiquan point and chongmen point’s immunoreactive product in acupuncture group is significantly lower than anesthesia group and control group, the di fference statistically significant; compare to the control group, anesthesia group of acupoints on immunoreactive product of gray-scale values more lower in anesthesia group, the difference was not statistically significant.Group2:the gray-scale value of the ncupoint’ simmunoreactivc product in acupuncture group is signi ficantly lower than non-points and the opposite side points, the difference statistically significant; compare to the opposite side points, the immunoreactive product of gray values more lower in non-points. the difference was not statistically significant.Group3:the immunoreactive area of jiquan point and chongmen point in acupuncture group is significantly higher than the corresponding points in anesthesia group and control group, the difference statistically signi ficant. Compared to the control group, the acupoints on immunoreactive regional immunization is higher in anesthesia groups, the difference was not statistically significant.Group4:the immunoreactive area of acupoint in acupuncture group is significantly higher than the non-points and opposite side points. Compared to the opposite side points, the immunoreactive area is higher in non-points area, the difference was not statistically significant.Group5:the CytC protein expression level of jiquan point and chongmen point in acupuncture group is significantly higher than the anesthesia group and control group, the difference statistically significant. Compare to the control group, the CytC protein expression levels of point area is higher in an esthesia group, the difference was not statistically significant.Group6:the CytC protein expression level of point area in acupuncture group is significantly higher than non-point area and the opposite side point area, the difference statistically significant. Compared to the opposite side point area, the CytC protein expression levels is higher in non-acupoint, the difference was not statistically significant.Conclusion:Electro-acupuncture can improve the positive rates of mitochondrial cytochrome c in accupoints and the cytochrome c protein expression levels, provide a new idea in clinical acupuncture Meridian energy transmission.