Observation The Influence Of Very Long Chain AcylCoA Dehydrogenase Deficiency By In Vitro Probe Assay Under Heat Stress And Studying Of The Intervention Effects Of Lycium Barbarum Polysaccharides

Objectives To investigate the influence effects of lycium barbarum polysaccharides (LBP)on Skin Fibroblast of very long chain acyl—CoA dehydrogenase deficiency (VLCADD) byin vitro probe assay (IVP) both under heat stress（40℃）and studying the effects of lyciumbarbarum polysaccharides (LBP) both physiological temperature（37℃）and heat stress（40℃）.Methods Human skin fibroblasts from very long chain acyl—CoA dehydrogenasedeficiency and normal control were cultured in medium loaded with unlabelled palmitic acidfor96h both at37℃and at40℃with or without LBP（0.4mg/ml）,and the quantitative ofin-vitro acylcarnitine profiling (AP) in the medium was analyzed by using tandem mass andstudied the effect and related mechanism.Results Compared with physiological temperature（37℃）, acyl-carnitine (C2) increasedsignificantly (P＜0.05）, while long-chain acylcarnitines increased significantly (P＜0.05）,including palmitic carnitine(C16), dodecanoylcarnitine(C12) and tetradecanoglcarnitine(C14) under heat stress. Howerever, long-chain acylcarnitines such as C12,C14and C16decreased significantly (P＜0.05） and acyl-carnitine (C2) increasedsignificantlyafter adding LBP both at37℃andat40℃.Conclusion1. Heat stress worsen the oxygen process of skin cell of VLCADD, which increased theaccumulation of the long chain acyl-carnitine such as C12,C14and C16.2. LBP decreased the long chain acyl-carnitine of human skin cell of VLCADD, and decreased the long chain acyl-carnitine accumulation that had the positive effect to VLCADDusing in vitro probe assay.The mechanism maybe associated with its effect in antioxidationstress.