The Dynamic Change Of GPⅡb/Ⅲa、GPⅠb/Ⅸ/Ⅴ In The Serum Of Rats Which Were Received High-voltage Electrical Burn And The Intervention Effection Of Pentoxifylline(PTX)

Objective: Through the pre-experimental research work, We observedthat specimens mesenteric, intestinal mucosa, kidney cortex, liver, gastricmucosa, deep fascia, the soft meninges and other microcirculation ofexperimental animals changes,after the general voltage electric burns.We canfind not only the high-voltage caused by peripheral tissue microcirculationdisturbance, but also to distant organs, caused by microcirculation disorders,leading to systemic microcirculation, causing body damage can be observedthat the performance of micro-Yan Bi leukocyte rolling and adhesion, whitesmall thrombus mobility and erythrocyte aggregation phenomena. SomeExperiment has proved that platelet GlucoproteinⅡ b/Ⅲa(CD41/CD61) andGlucoprotein Ⅰb/Ⅸ/Ⅴ(CD42) taken an important role on assemble andadhesion of platelet and thrombosis. The plateletGlucoproteinⅡ b/Ⅲa (CD41/CD61) ascend and Glucoprotein Ⅰb/Ⅸ/Ⅴ(CD42)lower is an important symbol to actvation of platelet. We designed thisexperiment aims to observe the high voltage electric burn plateletGlucoprotein Ⅱb/Ⅲ a and Glucoprotein Ⅰb/Ⅸ/Ⅴof the continuous changes involtage electric burn to further explore the mechanism microcirculationdisorder, and pentoxifylline on serum platelet Glucoprotein expressionintervention, These microcirculatory disturbance after burn injury preventionand treatment for high-voltage and provide a theoretical basis for the greatestpossible reduction in patient mortality, improve survival rate.Methods：1. Group:90healthy adult male S-D rats are parted into three groupsrandomly. There are experiment group by high-voltage electrical burned(experiment goup), high-voltage electrical burn PTX reatment group (treatment group) and pretend high-voltage electrical burn group (controlgroup),30rats of each group. The phase of these groups are parted into sixdifferent phases, ahead(15min)(T0), immediately(with in5min)(T1),1h(T2),2h(T3),4h(T4),8h(T5),10rats of each group.2. Preparetion: Number and weigh the rats,then shed the rats’feather ofleft upper limbs and light legs,and record them.The concentration ofexperimental drug required by eperiment’s dubbed.3. Duplicate the high-voltage electric burn model: Anaesthetic rats ofelectrical burn experiment group and PTX treatment group are burned by the1,000volts high-voltage electric system for3seconds,they are duplicatedfull-thickness electrical burn with body tissues that touch with the electrodes,we immediately inject PTX(50mg/ml) to rats of treatment group throughabdomen, normal sodium to the experiment group and the control group.Theprogress of control group is the same as the electric burn group,but there isn’telectric current in the body of control group rats.4. Collection and detection:In different time phases open pectoral cavitytake suction blood about5ml from rats’ heart and fractionate4ml and1ml,the former centrifuge with3000r/min about10minutes,suctionsupernatant,and then preserve in ultra low temperature freezer.5. The serum content of platelet Glucoprotein Ⅱb/Ⅲa andGlucoprotein Ⅰb/Ⅸ/Ⅴof the rats were measure by enzyme-linkedimmunosorbent assay(ELISA) at different time phases during early electricalburn and effect of PTX to the both.6. Analysis of Statistics: statistically analysis all the data withSPSS16.0.all the dates should be expressed with x±s.we apply one-factoranalysis of variance(ANOⅤA) among groups.It has significant statisticallymeaning if p<0.05.Results:the changes of platelet GP Ⅱ b/Ⅲa and GP Ⅰb/Ⅸ/Ⅴin the serum1. The serum levels of platelet GPⅡ b/Ⅲ a and GPⅠ b/Ⅸ/Ⅴhaven’tobviously change within the control group.(p>0.05)2．From T1to T4phase, the experimental group of early-phase serum platelet GP Ⅱb/Ⅲ alevels higher than T0phase and control groups’(p<0.05),the serum platelet GP Ⅱ b/Ⅲa levels on T5phase have no obviouslychange among the control group、experimental group and T0phase（p>0.05）.GPⅡ b/Ⅲa up to the highest level at once after high-voltage electricalburn,and then,with time past, levels of GP Ⅱ b/Ⅲ abecome lower andlower,until T5phase back to the levels of before the high-voltage. From T1to T2phase, the experimental group of early-phase serum platelet GP Ⅰ b/Ⅸ/Ⅴlevels are lower than T0phase and control groups’(p <0.05),the serumGPⅠ b/Ⅸ/Ⅴlevels on T3、T4、T5phase have no obviously change among thecontrol group、experimental group and T0phase （p>0.05）. GP Ⅰ b/Ⅸ/Ⅴupto the lowest level at once after high-voltage electrical burn,and then,withtime past, levels of GP Ⅰ b/Ⅸ/Ⅴbecome higher and higher,until T3phaseback to the levels of before injury.3. From T1to T4phase, the treatment group of early-phase serum plateletGPⅡ b/Ⅲa levels are higher than T0phase and control groups’(p <0.05),butlower than the experimental group in the same phase (p <0.05),the treatmentgroup serum platelet GPⅡ b/Ⅲ alevels on T5phase have no obviously changeamong the control group、experimental group、treatment group and T0phase（p>0.05）. From T1to T2phase, the treatment group of early-phase serumplatelet GPⅠ b/Ⅸ/Ⅴ levels are lower than T0phase and control groups’(p<0.05),but higher than the experimental group in the same phase (p<0.05).the treatment group serum platelet GPⅠ b/Ⅸ/Ⅴ levels on T3、T4、T5phase have no obviously change among the control group、experimentalgroup、treatment group and T0phase（p>0.05）.8-hour-phase electric shockgroup and treatment groups decreased between the two groups, but nosignificant difference (p>0.05).Conclusion:1After high-voltage electrical burned,serum levels of plateletGPⅡ b/Ⅲa has increased and GPⅠ b/Ⅸ/Ⅴ has decreased,with the timepast,the both can back to the levels before injury,which indicate plateletactivate,blood stick,flow of blood slow,then microcirculatory disorder. 2The treatment group levels of GPⅡ b/Ⅲ a are lower than experimentalgroup,still higher than control group.On the contrary, the treatment grouplevels of GPⅠ b/Ⅸ/Ⅴ are higher than experimental group,still lower thancontrol group.The result indicate that pentoxifylline(PTX) can lessen the levelof platelet activation,reduceing degree of blood stick.So PTX can alleviatemicrocirculatory disorder.