Long-term Effects Of High-voltage Electric Burn On Serum PDGF And TPO In Rats And Intervention Of Xuebijing And Ulinastatin

Objective:To investigate the long-term effects of high-voltage electric burn on serum PDGF and TPO in rats and the intervention effects of Xuebijing and Ulinastatin.Methods:A total of 350 clean grade SD rats were randomly divided into the sham group,electric injury group,saline group,Xuebijing group and ulinastatin(UTI group).Each group consisted of 70 rats.In accordance with the random number table method,it is divided into seven time groups on average:15 minutes before electrical injury,1 hour,8hours,24 hours,48 hours,72 hours after electrical injury and 1 week after electrical injury.It is 10 people.Using the left forelimb as the current inlet and the right hind limb as the outlet,the rats were electrically shocked with 2KV high voltage and(1.92±0.24)A current using the experimental electric shock system and transformer.Each group was injected intraperitoneally with 2 mL/kg physiological saline in time.Blood Betamine 10ml/kg,1%UTI(2×10~4u/kg)2mL/kg.Blood was collected from the heart by 5-7 ml,serum was separated,and the contents of PDGF and TPO were detected by ELISA double antibody sandwich method.An analysis of variance in the design of the data factorial design.Results:1.At 1h,8h,24h,48h,72h,and 1w after injury,the serum level of PDGF was higher in rats with pure electrical injury than in the pseudogroup(P values were less than 0.05)and 15min before electrical stimulation(P values were all less than 0.05),There was no statistical difference between the phases of the sham injury group(P value greater than 0.05),and the electric injury group was significantly higher than the other phases at 8h and 24h after electricity,and showed a"double peak"trend.Serum PDGF levels in Xuebijing group and ulinastatin group were significantly lower than those in electric injury group and saline group(P values were less than 0.05),and both of them could inhibit secondary peak of PDGF after 24h.2.At 1h,8h,24h,48h,72h,and 1w after injury,the serum TPO levels in rats with simple electrical injury were higher than those in the pseudogroup(P values less than 0.05)and 15 min before electrical stimulation(P values less than 0.05).The serum TPO levels in the Xuebijing group and the ulinastatin group were significantly lower than those in the electric injury group and the saline group(P values less than0.05).There was no statistical difference between the phases of the sham injury group(P value greater than 0.05),and the electric injury group was significantly higher than the other phases at 8h and 24h after the electricity,and showed a"double peak"trend.Conclusion:1.The high piezoelectric burns can increase the concentration of PDGF and TPO in the blood of rats and reach the peak at8h after injury.It increases again at 24h after injury and decreases after48h.This may be related to wound infection at 24h after injury.2.High-voltage piezo burn can stimulate the activation of intracellular PDGF and TPO signal transduction pathways,enhance its regulation of related gene transcription,participate in intracellular inflammatory reactions after high-voltage electric burns,and affect the body's microcirculation.The specific mechanism remains to be further studied.3.Early application of Xuebijing and UTI treatment after high voltage electric injury can significantly reduce the content of PDGF and TPO in serum,indicating that Xuebijing and UTI can reduce the levels of PDGF and TPO after high-voltage electric burn,reduce the inflammatory reaction,and improve microcirculation.UTI's anti-inflammatory and improved microcirculatory effects may be better than blood-neutral in the shock phase.However,the comparison of its specific effects and mechanisms need further exploration.