Studies On Chemical Constituents Of The Aerial Part Of Clematis Manshurica Rupr

In this paper, the northeast wire Ephraim (Clematis manshurica Rupr.) theground part (stem) of chemical composition, which is expanding Chinese medicineWeiLingXian drug resources, reuse and offer scientific basis.In this article, the method of continuous reflux extraction clematis thenortheast part of Ephraim effective components, with column chromatography,preparation methods, such as type thin layer of the earth wire the northeastEphraim active ingredients of the purification, and by high performance liquidchromatography, infrared, ultraviolet, mass spectrometry, NMR spectroscopymeans such as identified six compound structure, it is respectively: betasitosterol (Ⅰ), oleanolic acid (Ⅱ), anemonin (Ⅲ), chlorogenic acid (Ⅳ),coffee acid (Ⅴ) and ferulic acid (Ⅵ), of compounds (Ⅳ),(Ⅴ),(Ⅵ) for thefirst time in the stem leaf found; Using naphtha and soxhlet extraction deviceis the volatile components and fat oil extraction of the ingredients, andanalyses the GC-MS analysis, identified the24kind of volatile organic compoundsand12kinds of fat oil component; Use contents were determined by HPLC methodfor the northeast part of the earth wire Ephraim the content of chlorogenic acidfor1.36mg/g; Water extraction and alcohol heavy law to extract pointy endspolysaccharide, using ultraviolet spectrophotometry were of polysaccharide, getthe contents of polysaccharide is1.6mg/g; Through the icp-ms to its containtrace elements for testing, test results to contain8kinds of necessary traceelements, including chromium (Cr), zinc (Zn), copper (Cu), nickel (Ni) therelative content of higher; Using automatic amino acid analyzer of amino acidwas studied, it contains16essential amino acids, total amino acids for22.92ug/g.The results of the study in the northeast part of the earth wire the effectiveuse of Ephraim provide the scientific basis for expanding WeiLingXian laid thefoundation of the medicinal resource, also make the ground part can reuse.