The Expression And Significance Of The Acid Phosphatase In Cervical Intraepithelial Neoplasia And Cervical Cancer

[Background] Currently, cervical cancer is the second most common malignant tumorsin the female. Each year, there are about500,000new cases of cervical cancer aroundthe world and more than25million patients die from cervical cancer a year. More than80percent occur in developing countries, in these countries, cervical cancer incidenceup to80/100000. Cause of cervical cancer has not yet fully clear. The occurrence ofcervical cancer are thought that is related to early marriage, early childbearing, prolific,chaotic sex life and other factors. There is a clear correlation between the occurrence ofcervical cancer and high-risk human papilloma virus (HPV) persistent infection. Almostall patients with cervical cancer can be detected out DNA of high-risk types of HPV.The occurrence of cervical cancer usually takes through the process of cervicalintraepithelial neoplasia(CIN), and it is generally believed that the process of cervicallesions from CIN to cervical cancer needs5-10years. If the precancerous lesions orcervical cancer can be discovered early, the cure rate of patients with cervical cancercan be greatly improved. Currently, the screening of cervical cancer in clinical primarilyby cytology and virology testing, and there are still some patients missed to diagnosis.So to find a new biological marker in a cervical lesion become to an important goal forimproving the rate of diagnosis of cervical lesions more accurately.Acid phosphatase is a hydrolase which can hydrolyze phosphate in an acidicenvironment. It has five subtypes, and which related to human among them are ACP1,ACP2, ACP3and ACP5. ACP3has been seen as specific tumor markers for prostatecancer at the earliest, which has an important significance in the diagnosis and detectionprognosis of prostate cancer. ACP5mainly exists in osteoclasts, and its high expressionuniversally point out that malignant tumor happened to the bone. However, recent studies show that ACP not only exist in human prostate tissue, high expression ofacid phosphatase in bone tumors, breast cancer and other tumors are also presented.Some reports in the literature show that lysosomes ACP activity increased in abnormalcervical squamous cell, but the ACP expression in cervical squamous cell carcinoma isreported rarely and the subtypes uncertain. Therefore, our subject wants to detect theexpression of ACP in the cervical lesions in different aspects. We hope to learn thesignificance in the diagnosis of cervical cancer through discussing the abnormalexpression or activation of ACP,and point out a new diagnostic marker which canimprove the rate of early diagnosis of cervical cancer.[Objective] Through detecting the expression of ACP in normal cervical epithelium,cervical intraepithelial neoplasia and cervical carcinoma in different ways, we point outa new diagnostic marker which can improve the rate of early diagnosis of cervicalcancer.[Methods]1）RT-PCR was used to determine the expression of four subtypes of ACP in normalcervical epithelium, cervical intraepithelial neoplasia and cervical carcinoma, andscreen the ACP subtype which relate to cervical cancer2）Western blot were used to examine the expression of ACP1in epithelial tissue ofnormal cervical epithelium and cervical lesions3）IHC was used to examine the differential expression of ACP1protein in normalcervical epithelium, cervical intraepithelial neoplasia and cervical carcinoma4）To examine the differential expression of ACP1in cervical squamous cellcarcinoma Siha, and C33A cell lines in different ways5）IHC was used to examine the differential expression of ACP1protein in the tissueof normal cervical glands and cervical adenocarcinoma6）To examine the differential expression of ACP1in cervical squamous cell carcinoma Siha cell line and cervical adenocarinoma Hela cell line in different ways[Results]（1）RT-PCR shows that the expression of the ACP1is regular in normal cervicalepithelium, cervical intraepithelial neoplasia and cervical carcinoma. The expression ofthe ACP1is higher in Cervical lesions tissue than normal cervical epithelium. TheACP2and ACP5express irregular in the cervical tissues, and the ACP3do not expressin the cervical tissue（2）Western-blot shows that theACP1express in both normal cervical epithelium andcervical lesions epithelial, but in normal cervical epithelium it express little. The levelof the ACP1expression gradually increase as the cervical lesions increasing (p<0.05)（3）IHC shows that theACP1locates in the cervical epithelial cell cytoplasm, and thelevel of the ACP1expression gradually increase as the cervical lesions increasing(p<0.05)（4）The expression of ACP1mRNA and ACP1protein are no significant differencefrom the Siha and C33A cell lines (p>0.05)（5）The expression of the ACP1is significant difference from the tissue of normalcervical glands and cervical adenocarcinoma (p<0.05)（6）The expression of ACP1mRNA and ACP1protein are no significant differencefrom the Siha and Hela cell lines (p>0.05)[Conclusion] The expression of the ACP1is higher in Cervical lesions tissue thannormal cervical epithelium. The level of the ACP1expression gradually increase as thecervical lesions increasing. Increased expression of the ACP1in cervical lesions haveno relate to the HPV infection and the pathological types of cervical cancer. ACP1canbe seen as a new diagnostic marker of cervical cancer and precancerous lesions.