| Purpose:This project through the observation pulp core decompression combine extracture of safflower on rabbit steroid Steroid-induced Avascular Necrosis of the Femoral Head of model, expounds the combination therapy of steroid avascular necrosis of the mechanism.Method:(1) Experimental animal were divided into normal group and model group application ear marginal injection of horse serum plus intramuscular steroid method established in rabbits with steroid-induced avascular necrosis of femoral head in early models.(2)Made the surplus model into blank group, the control group, the pure pulp core reduced pressure group, the pure reduced pressure combined HSYA inject group (the "pure reduced pressure group and are combined treatment group). Blank group that did not make any treatment; According to the above method for control group made the individual,pure reduced pressure group only do pulp core decompression, combined treatment group do follow after decompression of the medullary cavity injection of safflower yellow pigment grain A hydroxyl group.To do the disposal of1,2weeks after respectively of the relevant indicators detection;(3) The ear venous blood sampling margin line hemorheology detection;(4) Under general anesthesia to each side to pressure inside the bone femoral head detection;(5) Take each side HE dyeing observe the femoral head bone trabeculae form; Immunohistochemistry methods were used observation vascular endothelial growth factor (VEGF) and vascular density count (MVD).Result:(1) Model group empty bone lacuna rate reached more than39%, compared with the normal group had significant difference, transmission electron microscope, normal bone cells, bone cells are mostly composed of morphological integrity, good function, model consisting of bone cell morphology function as normal group, the majority of bone cell shrinkage, poor function, the number of empty osteocyte lacunae, a combination of the above can be SANFH model was established.(2)The detection of blood rheology: intervention after the first batch of controls the whole blood viscosity (low cut, normal cut, high cut) and whole blood reduced viscosity (low cut) were higher than that of the blank group, there was significant difference (P <0.05); the combination group whole blood viscosity (low cut, normal cut, high cut) were lower than those of control group, there was significant difference (P<0.01); blood viscosity (low cut) lower than that of the control group, there was significant difference (P<0.05). The first batch of samples in the detection of simple decompression group whole blood viscosity (low cut) higher than that of the control group, there was significant difference (P<0.05), whole blood viscosity (cut, high cut) and the control group showed no significant difference (P>0.05), blood reduction viscosity (low cut) lower than that of the control group, there was significant difference (P<0.05); the second batch based detection of simple decompression group whole blood viscosity (low cut, normal cut, high cut) and the control group showed no significant difference (P>0.05), blood reduction viscosity (low cut) and the control group showed no significant difference.(P>0.05). The combination group whole blood viscosity (low cut, normal cut, high cut) and whole blood reduced viscosity (low cut) and the control group, no significant difference (P>0.05).(3) Pressure inside the bone test: pure reduced pressure group and combination group are lower than those of the control group within bone pressure, significant difference (P<0.05), and the combined treatment group within bone pressure, the effect is better than the lower pressure group, pure significant difference (P<0.05).(4) HE dyeing observation:joint reduced pressure group under mirror unit area of bony trabeculae form, quantity is better than the control group and pure reduced pressure group.(5)VEGF immunohistochemical:immunohistochemical method to detect femoral head cancellous bone specimens VEGF expression, the combined treatment group is better than the other group, significant difference (P<0.05).(6)MVD detection:immunohistochemical method to detect femoral head cancellous bone specimens microessel-density, combined treatment group than in control group and pure reduced pressure group, significant difference (P<0.05).Conclusion:(1) HSYA can reduce blood viscosity, the femoral head microcirculation provide favorable conditions;(2) HSYA can further reduce the pressure in the femoral head, the femoral head microcirculation provide favorable conditions to promote bone formation, promote new trabecular bone formation, HSYA huoxuehuayu effects of improving microcirculation and into the bone to provide the necessary nutrition support (;3) HSYA can promote within the femoral head and expression of VEGF in vascular endothelial cells of neonatal, microvascular density increased. |
