The Impact Of Swimming Training On The SOD, MDA,SDH Of Muscle In The Recovery Process Of Disused Muscular Atrophy Of Rats

Objectives:Through swimming training on rats of disused muscular atrophy, this article discusses the occurrence mechanism of the disused muscular atrophy and the influence of swimming on SOD、MDA、SDH of the disused muscular atrophy.Methods:Adopt40healthy male SD rats and divide them into5groups after a week’s adaptive feeding, namely the blank control group1(A), the natural control group2(B), tail suspension group (C), tail suspension+natural recovery group (D), tail suspension+swimming exercise group (E). Do not handle with group A and group B and let their rats have free diet. After tail suspension for two weeks, dissect the rats in group A and group C, takeout their soleus muscle, measure the weight of the rats, muscle wet weight and the cross-sectional area of muscle fiber to determine whether the model of disuse muscular atrophy is well established. At this moment, relieve the fixed group D and E, among which let the rats in group D make no movement and have free diet, get the rats in group E first have three days of free diet, and then go a two-day adaptive swimming for15minutes in each day. Then let the rats have a three-week swimming for6days each week and1day’s rest. In the first week, swimming for30minutes, swimming for60minutes in the second week, and90minutes in the third week. After the swimming exercise, measure their weight, dissect the rats in B、D、 E three groups, get out the soleus muscle, measure the wet weight of muscle, cross-sectional area of muscle fiber (CSA), the activity of superoxide dismutase (SOD), the density of malondialdehyde (MDA) and the activity of succinate dehydrogenase (SDH).Results:After two weeks’tail suspension, compared with rats in group A, the rats’ weight reduced30.15%in group C, the differences between the two groups have statistical significance (P<0.05); Compared with group A, the cross sectional area of soleus muscle fiber of the rats in group C dropped25.18%, the differences between the two groups also have statistical significance.Compared with group B, rats in group D recovered naturally by three weeks, the weight growth rate is79.11%, the difference is statistically significant (P<0.05); after three weeks’ swimming exercise, the growth rate of rats’weight in group E reached75.86%compared with the weight of group B, the differences are also statistically significant (P<0.05). Whereas the weight is4.23%higher in group E than that of group F, and this difference is not statistically significant (P>0.05).Compared with group B, after natural recovery by three weeks, the soleus muscle wet weight of rats in group D decreased19.58%, the difference was not statistically significant (P>0.05); the soleus muscle wet weight of rats in group E decreased15.82%, and compared with group B, the difference was also not statistically significant (P>0.05). However, compared with group D, the increase rate of soleus muscle wet weight in group E is5.08%higher, but this difference was not statistically significant(P>0.05).Compared with group B, after natural recovery by three weeks, the cross-sectional area of soleus muscle in group D decreased13.45%, the differences between the two groups were statistically significant (P<0.05); after three weeks’swimming exercise, the cross-sectional area of soleus muscle in group E decreased2.88%compared with that of group B, the differences were not statistically significant (P>0.05). Compared with group D, the cross-sectional area of soleus muscle in group E is12.21%higher, but this difference was not statistically significant (P>0.05).After natural recovery by three weeks, the activity of SOD in soleus muscle in group D was10.25%lower than that of rats in group B, the difference was not statistically significant (P>0.05); after three weeks’swimming exercise, the activity of SOD in soleus muscle in group E was2.88%higher compared with that of group B, the differences were also not statistically significant (P>0.05). The activity of SOD in soleus muscle in group E was14%higher than that of group D, which was statistically significant(P<0.05).After natural recovery by three weeks, the MDA density in soleus muscle in group D was31.42%lower than that of rats in group B, the difference was statistically significant (P<0.05); after three weeks’swimming exercise, the MDA density in soleus muscle in group E was11.05%higher compared with that of group B, the differences were not statistically significant (P>0.05). The MDA density of soleus muscle in group E was61.92%higher than that of group D, which was statistically significant (P<0.05).After natural recovery by three weeks, the activity of SDH in soleus muscle in group D was51.77%lower than that of rats in group B,the difference was statistically significant (P< 0.05); after three weeks’swimming exercise, the activity of SDH in soleus muscle in group E was1.17%higher compared with that of group B, the differences were not statistically significant (P>0.05). The SDH density of soleus muscle in group E was61.92%higher than that of group D, which was statistically significant (P<0.05).CONCLUSION:Three weeks swimming training can increase SOD and SDH activity of soleus muscle in the recovery process of disused muscular atrophy and also can increase the organism ability of cleaning free radical, and improve the aerobic capability, but had no significant effect on MDA concentration.