| Schiff bases is a class of organic compounds containing characteristic imine or azomethine group (-RC=N-). Due to their impressive catalytic and biological properties, Schiff base complexes have been widely used in catalysis, analysis and medication and so on. And, the copper Cu2+has a variable chemical valance, and is capable of participating in electron transfer and oxidation-redox process, the Schiff base-copper complexes play an important role in medical use and catalytic aspects, such as detection of AA, DA and UA. In this paper, the copper complex [Cu(Sal-β-Ala)(3,5-DMPz)2](Sal=salicylaldehyde, β-Ala=alanine,3,5-DMPz=3,5-Dimethylpyrazole) was prepared, and its biological toxicity on E. coli and Tetrahymena were investigated. After modifying the copper complex on the glassy carbon electrode(GCE), the [Cu(Sal-β-Ala)(3,5-DMPz)2]/singe-walled carbon nanotubes (SWCNTs)/GCE was prepared, and its electrocatalytic properties on biomolecules(Ascorbic acid, dopamine and uric acid) and organic pollutant compounds(dihydroxybenzene) were studied. The main content is as follows:1. Microcalorimetry was used to study the biological toxicity of [Cu(Sal-p-Ala)(3,5-DMPz)2] on the E.coli and Tetrahymena. The thermodynamics data, such as growth rate constant (k), Inhibitory ratio (I) and half inhibition concentration (IC50), have been obtained. The results showed that the complex had an obvious biological toxicity on E. coli and Tetrahymena. As the concentration increased, the metabolism of the E. coli and Tetrahymena were suppressed. The IC50of complex on E. coli and Tetrahymena were0.3and0.11mmol·L-1respectively.2. Cyclic voltammetry (CV) were used to electrodeposit [Cu(Sal-β-Ala)(3,5-DMPz)2] on the surface of SWCNTs/GCE electrode to fabricate [Cu(Sal-β-Ala)(3,5-DMPz)2]/SWCNTs/GCE. The modified electrode has a good electrocatalytic effect on AA, DA and UA. Under the optimal detection conditions, chronoamperometry was used to detect small biomolecules (AA, DA and UA) and obtained the following conclusions:(a) The detection linear range of [Cu(Sal-β-Ala)(3,5-DMPz)2]/SWCNTs/GCE to AA is5to535μmol·L-1and the detection limit is1.66μmol·L-1.(b) The detection linear range of [Cu(Sal-β-Ala)(3,5-DMPz)2]/SWCNTs/GCE to DA is10.00to210mmol/L and the detection limit is7.29μmol·L-1.(c) The detection linear range of [Cu(Sal-β-Ala)(3,5-DMPz)2]/SWCNTs/GCE to UA is1to86μmol·L-1and the detection limit is1.5μmol·L-1.(d) Differential pulse voltammetry (DPV) is applied for the simultaneous determination of DA, UA. The concentration of DA and UA and the peak current of oxidation were in linear relationship in the experimental concentration range. These results demonstrated that the modified electrode has a high sensitivity and a wide linear range for the determination of AA, DA and UA.3. The modified electrode ([Cu(Sal-β-Ala)(3,5-DMPz)2]/SWCNTs/GCE) was used to detect catechol(CT) and hydroquinone(HQ). The modified electrode has a good electrocatalytic effect on CT and HQ. Under the optimal detection conditions, DPV was used to detect CT and HQ separately and simultaneous. The results are as follows:(a) The detection linear range of [Cu(Sal-P-Ala)(3,5-DMPz)2]/SWCNTs/GCE to CT is5-69.5and69.5-215μmol·L-1and the detection limit is3.5μmol·-L-1.(b) The detection linear range of [Cu(Sal-β-Ala)(3,5-DMPz)2]/SWCNTs/GCE to DA is5-70and160-370μmol·L-1and the detection limit is1.46μmol·L-1.(c) The concentration of CT and HQ and the peak current of oxidation were in linear relationship in the experimental concentration range. These results demonstrated that the modified electrode has a high sensitivity and a wide linear range for the determination of CT and HQ. |
