The Key Technology Of Cordyceps Militaris Cultivation

Cordyceps militaris is a kind of precious edible fungus. It is an ideal health food, whichnot only contains rich nutrients, but also has many functions such as hypotensive,hypoglycemic, antifatigue, antineoplastic, enhances human immunity and so on. Therefore,researching the key cultivation techniques of Cordyceps militaris has great significance to thedevelopment and utilization of Cordyceps militaris.This paper mainly studied the following four contents: cultivation substrate, severalenvironmental factors, some essential parameters and validation identification of commonpathogenic bacteria on Cordyceps militaris cultivation. The main research output wasfollowing:1. Using stroma yield, stroma density, stroma length, biological efficiency and utilizationrate of medium as indexes, measuring above indexes on different nutritional conditions,establishing the mathematical models between different nutritional conditions and aboveindexes, it researched the effect of different carbon source, nitrogen source, mineral salt,ammonium ferric citrate and triacontanol on development and growth of Cordyceps militaris.The results showed that Cordyceps militaris could use monosaccharide, disaccharide,polysaccharide, but when sucrose was used as the carbon source, the growth of Cordycepsmilitaris was best, its best concentration was7.2g/L. Cordyceps militaris had a wide use ofnitrogen source, of which milk was the optional nitrogen source and its optional concentrationwas43.9mL/L. In selected mineral salt, potassium chloride had the most obvious function onCordyceps militaris, its best concentration was6.1g/L. In addition, the proper amount ofammonium ferric citrate and triacontanol were conducive to the development and growth ofCordyceps militaris, and their optional concentrations were0.4g/L and1.0mg/L.2. By establishing mathematical models, it studied the temperature on the Cordycepsmilitaris mycelium growth rate and the effect of the initial pH of medium on the growth ofCordyceps militaris. The results showed that Cordyceps militaris in18℃to28℃within allcould grow, when the temperature was23.7℃, the mycelial growth rate was the largest, itsmycelium growth rate was4.1mm/d. Medium which was too acid or alkali was hard togrowth and the metabolites accumulation of Cordyceps militaris. When the intinal pH was7.4,the all indexes of Cordyceps militaris reached the best. 3. It studied the medium usage, material water ratio and inoculation size on thedevelopment and growth of Cordyceps militaris and the effect of stroma culture time on thecordycepin content of Cordyceps militaris, meanwhile, using the method of quadraticorthogonal rotary design, it studied the medium usage and material water ratio on thedevelopment and growth of Cordyceps militaris. The results showed that when medium usagewas350g/box, material water ratio was1:1.5, inoculation size was74mL/box, Cordycepsmilitaris stroma density was the biggest, production cycle was shortest, utilization rate ofmedium was highest, the yield was largest. Beside, with the extension of stroma culture time,cordycepin content increased rapidly in the late cultivation. It showed that cordycepin wasmainly synthesis at the later grow stage of Cordyceps militaris.4. Using sequence analysis method of16S rDNA and ITS, combining withmorphological characteristics of bacterial and fungi, as well as, physiological and biochemicalidentification of bacteria, it studied three bacterial and two fungi which caused disease in thecultivation of Cordyceps militaris. The results showed that X1~X3and Y1~Y3have highsimilarity with Flavobacterium chungangense, Flavobacterium chungangense,Janthinobacterium lividum, Fungal sp. YX-2, Calcarisporium sp. LB2012-1, Gibberellaintermedia AB92, and speculated that may be for their relatives. It has important guidingsignificance in preventing and controlling common disease on artificial cultivation ofCordyceps militaris.