| Objective：By simulating different+Gz value exposure on the gastric mucosal injury inrats, and to investigate the potential mechanism of gastric mucosal injury may occurafter exposure to high+Gz value., we established animal centrifuge simulation+Gzexposure rat model, and observed observation the different+Gz value of exposing theextent of stress-induced gastric mucosal injury in rats, the level of gastric juiceepidermal growth factor (EGF) and gastric gastrointestinal hormone prostaglandin E2(PGE2) and somatostatin (SS) levels in rats.to explore the possible mechanisms of therat gastric mucosa in rats under simulate different the+Gz value of exposure.This mayprovides provide a theoretical basis for the pilots of peptic gastric mucosal diseaseprevention.Methods：40male Wistar rats were randomly and equally divided into four groups,namely the control group:+5Gz value exposure group,+10Gz value of the exposedgroup and repeated exposure group (n=10), animal centrifuge simulation+Gz exposurepreparedThe mouse+Gz exposure model.Rats were fasted for24h, the control rats:+1Gz value analog+Gz exposure exposure5min; to+5Gz exposure group:+5Gzcontinuous exposure5min; the+10Gz exposed group:+10Gz continuous exposure5min;continuous exposure group:+5Gz exposure for1.5min,+10Gz exposure2min+5Gzexposure for1.5min.The specimens were generally observed in rats rats perexperimental animal centrifuge. The experimental parameters include three parts:(1)gastric mucosal injury related parts: the degree of mucosal injury the Guth standard method to calculate the gastric mucosal damage index, mucosal structure of themicroscope to observe the degree of gastric mucosal injury of rat stomach.(2) byradioimmunoassay(RIA).of gastric juice epidermal growth factor EGF content.Relevantpart:.(3)gastrointestinal hormones were measured by radioimmunoassay(RIA) ratmucosal prostaglandin E2(PGE2) and somatostatin (SS) level.Results：(1) The general observation: the gastrointestinal mucosa of rats in the controlgroup and the group of+5Gz smooth and complete: a small amount of food residue inthe stomach, the gastric mucosa is smooth, uniform pale pink, no bleeding, erosion andulceration.The+10Gz experimental group and the continuous exposure to theexperimental group of rats with the naked eye observation fundic, antral and duodenalmucosa were seen scattered in small ulcers and bleeding spots, the flushing seen aftergastric mucosal surface diffuse edema, seen widely distributeddifferent sizes of erosion,hemorrhage, only the bottom of the gastric body and gastric mucosa obvious, somemucosal hyperemia exfoliation.(2)+Gz value exposed to gastric mucosal damage index:Compared with the control group (+1Gz values exposed in rats),+5Gz little gastricmucosal injury exposed rats,gastric mucosal damage index (vs0.00±0.00,0.35±0.21,P>0.05); compared with the control group, the+10Gz exposed rats gastric mucosalinjury significantly increased the gastric mucosal damage index (8.56±0.32vs0.00±0.00, P <0.01); continuous exposure group to the degree of mucosal injury comparedwith the control group (10.33±0.41vs0.00±0.00, P <0.01) significantly increased.+10Gz exposure group comparison:+5Gz exposure group with a significant differencebetween the rats (8.56±0.32vs0.35±0.21, P <0.01); continuous exposure group and+5Gz exposure group compared with rats have significantlydifference (10.56±041vs0.35±0.21, P <0.01). Compared continuous exposure group of rats and+10Gzexposure group (10.56±041vs8.56±0.32, P=0.028) have significantlydifference.(3) to simulate different+Gz exposure to the organizational structure of the rat gastric mucosa:,+5Gz exposed rats compared with the control group,+10Gz exposure groupand continuous exposure group rat gastric mucosa structural damagesignificanthistological lesions invade the mucosa of full-thickness, organizational structureseverely damaged.(4) Different+Gz value exposure of rat gastric juice EGF content:Compared with the control group (+1Gz value exposure),+5Gz exposure of EGF inthe gastric juice of rats decreased (P <0.001);compared with the control group (+1Gzvalue exposure),+10Gz exposure group, continuous exposure of rats gastric juice EGFdecreased significantly (P <0.001).(5) different+Gz values exposed rat gastrointestinalhormone levels: compared with the control group (+1Gz value exposure), with the+Gzexposure value increased in rat gastric mucosa somatostatin (SS) content meansignificantly higher compared to the control group and+5Gz rat gastric mucosa in SScontent showed no significant difference (P>0.05)+10Gz group compared withcontinuous exposure to rat gastric mucosa in SS content P <0.05the rest of the twogroups was compared both P <0.01. Compared with the control group (+1Gz valueexposure), with the+Gz exposure values increased content of rat gastric mucosaprostaglandin E2(PGE2), mean decreased each group, the control group, and+5Gz ratsgastric mucosa prostate hormone content of E2(PGE2) compared to no significantdifferences (P>0.05), the rest of the two groups was compared contrast twenty-twowere statistically significant (P <0.05).Conclusions：(1) with different+Gz values in rats exposed to the degree of damage tothe gastric mucosa.+Gz exposure value increases, the more severe the degree of gastricmucosal injury.(2) The higher+Gz value exposure and repeated exposure to lowerlevels of gastric juice EGF, the greater the degree of gastric mucosal damage in rats rats.(3) Simulate different+Gz values exposed rats can increase the degree of reaction ofthe gastric mucosa, the gastrointestinal hormone content of the rat gastric mucosa levelchange. The exposure of the low the+Gz value of (+5Gz value), the decline in rat gastric mucosal PGE2content increased the extent and the gastric mucosa SS contentwere smaller, to a lesser extent of gastric mucosal damage. With the+Gz exposed thedegree of increase in the value of the rat gastric mucosa PGE2content decreasedsignificantly higher content of SS may cause acute gastric mucosal injury worse.Simulate different+Gz values in rats exposed to acute exacerbation of gastric mucosadamage extent, not only performance is also reflected in the level of influence gastricjuice EGF expression in gastric mucosal damage index and histological changes in thegastric mucosa;,changing the content of the gastric mucosa of the gastrointestinalhormone defense capability and reduce resistance to damage. |
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