| Objective:Lesion before clinical observation in patients with mammary gland illhyperplasia, breast cancer, breast cancer in the breast tissue the expression of survivin andcaspase-8. Animal experimental study on emulsion block elimination of soup in thetreatment of disease of mammary gland hyperplasia in rats and against apoptosis genes(survivin) and promoting apoptosis (caspase-8), the influence of,From the aspects ofpromoting apoptosis research the mechanism of traditional Chinese medicine treatment ofdisease of mammary gland hyperplasia.Methods:Clinical research to select patients with mammary gland ill hyperplasia,breast lesion,20cases of breast cancer before wax block specimens,Immunohistochemicalmethod to detect survivin caspase-8expressed in various pathological types in theorganization.Experimental research on100Vister female rats were randomly divided intoblank group and the control group, breast piece soup group, milk group, tamoxifen group,each group of20.Other groups of animals except the blank group were injected estradiolbenzoate0.5mg/kg/d,25days in a row, progesterone4mg/kg/d, made mould for fivedays.Will be made after the success of the mould blank group and the control group rats to1ml/100g/d physiological saline;Milk block elimination soup group to the breast blockelimination soup2ml/100g/d;Breast milk to add group to gain better solution is1.5g/100g/d; Tamoxifen group to tamoxifen solution of0.2mg/100g/d,Treatment for4weekscontinuously.Mammary glands in rats, through HE staining, immunohistochemicalmethod,To observe morphology of mammary gland,And cell antiapoptotic gene (survivin)and promote the expression of apoptosis gene (caspase-8).Results：(1) of survivin in front of the mammary gland hyperplasia, breast lesions and the positive expression rate of breast cancer (%) were88.20±3.21, and90.40±3.01,and85.70±3.39，There are significant differences (p <0.05);Caspase-8in front of themammary gland hyperplasia, breast lesions and the positive expression rate of breastcancer (%) were76.75±3.51, and74.30±3.10, and72.0±53.09;There are significantdifferences (p <0.05);(2) the blank group, control group of rats, breast piece soup, milkincreasing group, tamoxifen group of breast tissue morphology scores were1±0.00, and3.38±0.39, and1.55±0.22, and2.1±0.19, and1.33±1.43, Compared with blank group,control group with significant difference (P <0.01),That building success;Milk blockelimination soup, milk is added with the control group, tamoxifen group compare, haveobvious difference (P <0.01).(3) the blank group, control group of rats, the milk soup,milk increasing group, tamoxifen group of survivin expression rate were89.55±0.69, and92.20±1.51, and90.95±0.76, and91.85±0.74, and91.65±1.09Caspase-8expressionrate (%) were74.90±0.91, and71.75±1.56and73.30±0.98,and72.30±1.08, and72.600±.26.Milk block elimination soup, milk is added with the control group comparedthe expression of survivin and caspase-8, have obvious difference (P <0.01),Milk blockelimination soup with tamoxifen group compared the expression of survivin and caspase-8, has a difference (P <0.05).Conclusion：Clinical studies show that the expression of survivin levels the lowest inthe mammary gland hyperplasia,Reached the highest breast cancer lesions before lowerbreast cancer;Caspase-8with the expression level of hyperplasia of mammary glands-lesion before breast cancer-breast cancer lesion degree of increase and decrease.Animalexperiment study shows that by histopathological form and survivin in the control groupand blank group, the comparison of caspase-8,Estrogen, progesterone induced jointhyperplasia of mammary gland of rats model building success;Application blockelimination soup treatment rat mammary gland hyperplasia,In an organization can reducethe pathological changes of mammary gland hyperplasia degree on the morphology,At themolecular level can be cut in the expression of survivin and raised caspase-8. |
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