Study On Quality Standard Of Herba Patriniae

Objectives:Patriniae Herba has an unique antibacterial and anti-inflammatory effect, which has attracted wide attention. But the lack of quality standards and the disorded application seriously restrict the development and application of Patriniae Herba. To provide theoretical foundation for the research of quality standards of Patriniae Herba, through establishing systematic quality evaluation methods; To provide experimental evidence distinguish the genuine medicinal materials and adultrant by establishing the fingerprint of Patriniae Herba.Methods:1. Preliminary identification was carried out on the self-collected Patriniae Herba. And index components in Patriniae Herba were identified qualitatively by TLC.2. The content of protocatechuic acid, chlorogenic acid and caffeic acid in Patriniae Herba was determined simultaneously by dual wavelength HPLC.3. The content of quercetin, Kaempferol in Patriniae Herba was determined simultaneously by HPLC.4. To establish an HPLC fingerprint method of Patriniae Herba and it was evaluated by cluster analysis, similarity analysis and principal components analysis.Results:1. Three batches of Thlaspi arvensis L. samples did not display blue fluorescent spots; Except the spots of28th sample was not clear in the corresponding position with the reference substance, TLC result showed that the herbs all displayed blue fluorescent spots with heterogeneous spot size and color. The inconsistency demonstrateed the content of chlorogenic acid in Patriniae Herba was different.2. It was the first time to establish HPLC methods to determinate the content of protocatechuic acid, chlorogenic acid and caffeic acid simultaneously, and the content of quercetin, kaempferol in the meantime. The methods were simple and rapid. The content ranges of the respective components were as follows:0.022～0.055%(sample NO.28、29and30were not detected),0.047～0.726%,0.005～0.038%(sample NO.28、29and30were not detected),0.006～0.105%,0.004～0.070%. The samples of Thlaspi arvensis L. did not have these components.3.33batches of samples were divided into two categories by cluster analysis. The first category was Thlaspi arvensis L., and the other one was Patriniae Herba. Chromatographic peak informations of recommended products (18batches) were analyzed by fingerprint analysis software. Mutual pattern was established from18batches. There were17common peaks while three peaks had been identified. To further similarity analysis, there was high similarity in the18batches of samples. The principal component analysis result had highly consistency with cluster analysis results.Conclusions:The method of quality evaluation is simple and rapid, which provides theoretical reference for the foundation of quality standard of Patriniae Herba. The fingerprint information is reliable and specifical. Patrinia scabiasaefolia Fischer ex Treviranus and P. villosa (Thunberg) Jussieu have high similarity and there are no special components, but the diference between Thlaspi arvensis L. and Patriniae Herba is significant combine the results of the determination of index components and fingerprint information. So the fingerprint estabolished can provide a reference for distinguishing Patriniae Herba and Thlaspi arvensis L.