| Ganoderma lucidum spore powder is powder and Ganoderma lucidum spore extract as raw material, Ganoderma lucidum filtered through water extraction, concentration, spray drying, and mixing process made powders with enhanced immune function. This paper established a method for the determination of polysaccharide peptide acids and nucleosides of Ganoderma lucidum, Ganoderma lucidum polysaccharides and detection methods have made improvements, Ganoderma lucidum polysaccharides peptide established method for the determination of which have not been reported at home and abroad, while the safety and enhance immunity function of the product were studied, Ganoderma extract containing spores quality materials and health food control provide experimental reference, but also laid the foundation for the development and utilization of such products. Its main contents include the following two aspects:1. The study on determination of active ingredients in GLS ganoderma powder Objective:To establish methods to determine Ganoderma lucidum polysaccharide, Ganoderma lucidum acid, nucleosides in GLS ganoderma powder. Methods:Using ultraviolet-visible spectrophotometry,using phenol-sulfuric acid determination of chromogenic method at485nm absorbance value, Water as solvent at60℃30min, after centrifugation was precipitated with ethanol, the mobile phase to Volume, to TSK4000pw (7.8mm x30cm) of the column, methanol:water=20:80as mobile phase at a flow rate of lml/min. The column temperature:35℃; injection Volumn:10μl, ELSD detectors conditions:drift tube temperature:55℃, sprayers heating mode, the gas pressure:45psi, HPLC-ELSD method for the determination of polysaccharide peptide cotent; With chloroform as solvent reflux extraction3times, each time1h, filtering to extract, and the filtrate dry dissolved in methanol, Agilent C18(4.5x150mm,5μm) chromatographic column, acetonitrile:1%acetic acid=3forth as mobile phase, wavelength258nm for detection, HPLC-PDA method for the determination of ganoderma lucidum acid A and C2content; With water as solvent ultrasonic extraction60min, use Symmetry C18(2.1x150mm,2.1μm) chromatographic column, methanol0.1%formic acid water as mobile phase, gradient elution UPLC-MS-MS cytosine, uracil, inosine, guanosine, adenosine5kinds of nucleoside composition on qualitative analysis, and with the Ultimate? AQ-C18(4.6x250mm,5μm) chromatographic column, methanol-0.01mol/L sodium acetic acid solution as mobile phase gradient elution, detection wavelength260nm, HPLC-PDA method determine the content of5kinds of nucleoside compounds. Results:Ganoderma spore powder dextran r=0.993, the average recovery rate was95.22%; polysaccharide peptide r=0.999, the average recovery rate was99.02%; ganoderma lucidum acid r=0.990-0.992, the average sample recovery rate was98.30-99.76%; Nucleoside compounds r=0.993-0.999, the average sample recovery rate was98.46-99.39%. Conclusion:The method of this paper to establish suitable for GLS ganoderma powder of Ganoderma lucidum polysaccharide, Ganoderma lucidum acid and the determination of the content of arginine method simple operation, good selectivity, repeatability and high recovery rate.2. Study on the function of enhancing immunity of GLS ganoderma powder Objective:To study the function of enhancing immunity of GLS ganoderma powder. Methods:Through animal experiments, evaluation methods of using the function of health food ingredients of GLS safety toxicology experiments and studies the immunity function. Results:Each dose group mice body weight, thymus index, spleen index, the mice delayed-type allergy induced by DNFB, carbon clearance ability in mice, mice were no statistical significance of NK cell activity; Samples, high dose group of mouse spleen lymphocyte proliferation induced by ConA enhanced obviously. Samples, the high dose group could increase serum hemolysin level; and can obviously promote the mice antibodies generated; Samples of high dose group mice chicken red blood cell phagocytosis rate of macrophage cell in abdominal cavity, the index increased significantly. Conclusion:According to the technical specification for health care food inspection and evaluation of health (2003), suggests GLS ganoderma powder can enhance immunity function. |
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