Extraction And Separation Of Cytisine From Thermopsis Lanceolate In The Study On Production Process

the research papers went for The more in-depth and meticulous research and analysis from Thermopsis lanceolate of cytisine detection method and production technology etc, getting some valuable experimental data and the experimental results were theoretically analyzed and summarized. The papers Elaborated on Extraction and separation of production process of cytisine from Thermopsis lanceolate, experimental data of its full and accurate reference can be provided for the industrial production.1Qualitative, quantitative cytisine analysis detection method about Sparteine mainly was went by thin layer chromatography, spectrophotometry and HPLC.Thin layer chromatography using of silica gel G thin layer plate, expansion agent was for ethyl acetate methanol (3:2:add a few drops of ammonia),and chromogenic agent was for improved Dragendorff reagent spray color or color of iodine vapor,TLC is a clear and a good separation effect, because the thin layer chromatography is not a necessary expensive equipment, simple operation, often as a method of line detection in production.High performance liquid chromatography with Inertsil C18(4.6mm×250mm,5um) column, the mobile phase was:Acetonitrile0.05%three triethylamine solution (10:90, V/V), the flow rate was1mL/min, detection wavelength is310nm, the injection volume was20u L, room temperature.Genistein content in the range of0.40-4.0μ G concentration and peak area values showed a good linear relationship.High performance liquid chromatographic method for the main methods of high resolution, fast detection and high accuracy seemed as sparteine quantitative detection.2The horse beansContent in different parts of different origin, are analyzed, the results showed that the different region cytisine content similar, Qinghai Guide the highest.The seeds of different parts of the genistein mainly distributed in Thermopsis lanceolate of the same plant, then stem was the lowest.thereby, the production should be used in horse bean seeds as raw material.3Single factor experiments test and orthogonal design L9(34) was studied on the methods and conditions of alkali from thermopsislanceolate flower extracting genistein, and The optimum conditions were as follows:20times volume of85%ethanol and85℃heat reflux extraction3times, each time2h.4The quality of adsorption and separation about alkali macroporous adsorption resin of genistein were selected, the optimum adsorption conditions:Extracts of genistein content in the range of3-6mg/mL, resin LSI010,the extraction ratio of1:15(g/mL), extract pH from4to5under the conditions of the exchange adsorption,3BV volume of75%ethanol elution, under the condition of cytisine the ratio can reach more than93%.5Study were isolated and purified by silica gel chromatography of cytisine, determining the optimum separation and purification conditions:Selection of Φ3.6cm X100cm silica gel column chromatography, the sample volume is10-15mg crude/mL silica gel, silica gel column used ethyl acetate methanol system for gradient elution, the purity of spent alkali products for more than80.4%of genistein, the recovery rate is75.8%.6According to the single factor test, orthogonal test, macroporous resin and also silica gel chromatography screening test results determined the separation and extraction, cytisine pilot process.7Industrial production is extracted from Thermopsis lanceolate that results were cytisine amplified:Genistein content in crude product was26.48%, the yield was5.31%, the content of cytisine boutique80.21%, the yield1.32%.