Influence Of Aerobic Exercise And Hydrogen-water To SOD&MDA Of NAFLD

Obejective: To study the influence of aerobic exercise and hydrogen-rich water toantioxidant ability of diet-induced obesity rat’s NAFLD.Methods: It is slected of106pcs clean and healthy male SD rats which are6-8weeks age. After one-week adaptive feeding, they are divided into two groupsrandomly: Control Group (CON),12pcs, is raised by the standard diet; High fat dietGroup (HFD),94pcs, is raised by high fat diet. After12weeks raising, CONgroup’s average weight is471.70±28.89g and HFD group’s average weight is537.60±41.81g. There is statistically significant difference, p<0.01. It means thatthe NAFLD model of fat rats is succeeded. Then we choose6pcs rats from CONgroup randomly,470.68±13.02g, and top50%rats from HFD group according tothe weight,554.51±28.22g. The new HFD group is divided into8groups randomlyand every group has6pcs rats: high fat diet control group (HC), high fat dietexercise group (HE), high fat diet hydrogen-normal-saline group (HHI), high fatdiet hydrogen-rich water group (HHO), ordinary diet control group (NC), ordinarydiet exercise group (NE), ordinary diet hydrogen-normal-saline group (NHI),ordinary diet hydrogen-rich water group (NHO). Exercise group is trained by themoderate intensity aerobic exercise treadmill test. Hydrogen-normal-saline group isinjuected by hydrogen-normal-salinethrough intraperitoneal injection.Hydrogen-rich water group drinks hydrogen-rich waterfreely.After8weeks interveneexperiment is finished, all the rats are fasting24hours andceliac anesthesia by chloralic hydras. Then liver and venous blood are taken andkept in the fridge to-80°C. Blood sugar, blood fat and SOD/MDA in liver will bemeasured. Lipid change of hepatic tissue will also be analyzed by oil red staining.Results:1. Moderate intensity aerobic exercise is better than diet control and hydrogen waterto lose weight, lower liver wet weight and liver index. Hydrogen water has noinfluence to the weight, but there is tendentious variationto liver wet weight andliver index. For this tendentious variation, the effect of intraperitoneal-injectedhydrogen-normal-saline is obviouser than free-drinking hydrogen-rich water’s.2. After the oil red staining and observation under the optical microscope, thehepatic tissue of exercise group is improved compared with ordinary diet group andhydrogen water group. It is almost same with CON group. But the hepatic tissue ofhydrogen water group is still existed the phenomenaof hepatic cords’ disorder,hepatocyte swell and fatty infiltration.3. During the experiment, all the rats’ blood sugar is within normal limits. It meansthat the regulatory function of insulin is not lost. ForTC/HDL/LDL, every group hasno statistically significant difference. But for TG, HC group is higher than CON group, p<0.01; HHO group is higher than CON group, p<0.05; both HE group andNHI group is lower than HC group, p<0.05; NE group is lower than HC group,p<0.01.4. After the intervene of aerobic exercise and hydrogen water, there is nostatistically significant difference for rat liver’s MDA concentrationbetweenexercise group and CON group, same to hydrogen water group.ComparedwithHC groups’MDA concentration, both HE group and NE group is obviouslowerthan HC group, p<0.01; HHI group and NHI group is also lower than HC group,p<0.05. There is no statistically significant difference between HHO/NHI group andHC group.5. After the intervene of aerobic exercise and hydrogen water, there is nostatistically significant difference for rat liver’s SOD contentbetween exercise groupand CON group, same to hydrogen water group. Compared withHC groups’SODcontent, both NHI group and NE group is obvioushigher than HC group, p<0.01; HEgroup and NHI group is also higher than HC group, p<0.05. There is no statisticallysignificant difference between HHO/HHI group and HC group.Conclusions:1. Aerobic exercise is able to lose fat rat’s weight, ease its fatty liver and improve itslipid metabolism.2. Both aerobic exercise and hydrogen water could enhance the antioxidantability ofrats’ liver.3. To increase the antioxidant ability of rats’liver, the effect ofintraperitoneal-injected hydrogen-normal-saline is better than free-drinkinghydrogen-rich water’s.