| Objective:This study aims to use nonalcoholic fatty liver rats swimming experiment to compare the improvement of histologic examination of nonalcoholic fatty liver disease in rats after exercise, whether PPAR alpha protein expression has consistenc with serum proteinlrisiny expression levelin liver tissue, explore and varifythat the movement of nonalcoholic fatty liver disease and related regulatory mechanism and the influence of the way.Methods:After adaptive feed for a week,50 in 50 male SD rats (20 g) weight to 200+, randomly selected from 10 fed chow (group C).Another 40 fed withonly high-fat diet group (H).After 12 weeks, nonalcoholic fatty liver disease model is observed, then a high-fat diet group (H) rats were randomly divided into three groups,10 in each group,:the control group (NC group) of nonalcoholic fatty liver disease, nonalcoholic fatty liver disease, aerobic exercise group (NE), one-time movement of nonalcoholic fatty liver disease group (NO groups) respectively.Among them, rats in NE, NO group underwent 6 weeks of swimming training,6 days per week. Regularly weigh weight of rats during the experiment, to observe the color of the rats, activity, diet, general situation.After the training, with 10% chloral hydrate 300 mg/kg) by intraperitoneal injection of wt anesthesia, take blood living abdominal incision in the abdominal aorta. Separation of serum, transfer-80 ℃ low temperature refrigerator save, in case.After taking blood, quickly to spin out the liver tissue integrity, achieve the liver pathological histology slice for the same parts in paraformaldehyde soaking 4 ℃ refrigerator, other parts with sterile tin mooring paper parcel after transfer to-80 ℃ refrigerator. Set aside.Using enzyme-linked immunoassay detection serum Irisin indicators, using Western Blot imprinting analysis method for determination of nonalcoholic fatty liver disease PPAR alpha amount of protein expression in rat liver.Results:(1) Normal liver group C form, bright red color, smooth surface, compared with group C, NC group with NO liver volume increased obviously, color is not bright red, cut fat, NE group has certain improvement.(2) Compared with group C, NC group of nonalcoholic fatty liver disease in rats serum Irisin changed little, NE group expression quantity significantly increased (P< 0.05), and the highest amount of Irisin protein expression group;NO group of nonalcoholic fatty liver rats serum Irisin expression quantity decreased obviously (P< 0.05).(3) Compared with group C, NC group of PPAR alpha amount of protein expression significantly reduced (P< 0.05);Amount of PPAR alpha protein expression and NE group significantly increased (P< 0.05), and the NE set of four groups of liver tissue protein expression level of the highest;NO amount of PPAR alpha protein expression group compared with group C.(4) Compared with group C, NE group of PPAR Irisin serum and liver protein expression were increased, but NO group, decreased.Irisin serum and liver tissue between the four groups of PPAR alpha is highly correlated to the amount of protein expression, r is 0.842. Conclusion:(1) Movement will promote the expression of Irisin Protein.(2) After a long time of aerobic exercise, a homogeneity on expression can be seen among serumlrisin, liver tissue PPARa protein rats with non-alcoholic fatty liver which indicates that serum Irisin expression level can influence the expression of liver tissue PPARa.(3) Aerobic exercise can have a very good impact on non-alcoholic fatty liver caused by high-fat diet. It has been testified that there is a way that can help change the situation of non-alcoholic fatty liver:exercise-irisin-PPARa-improving non-alcoholic fatty liver. |
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