Effects Of 8-week Swimming Exercise On Testicular Germ Cell Apoptosis In Rats With Nonalcoholic Fatty Liver Disease

Objective: To observe the effects of eight weeks swimming on germ cells apoptosis and the expression of Fas/Fas L, Caspase-3 of non-alcoholic fatty liver disease(NAFLD) rats, explore the possible mechanism of aerobic exercise intervene germ cell apoptosis of NAFLD rats. Methods: 50 adult male SD rats were randomly divided into control group(group C, n = 20) and model group(group M, n = 30); then group C was divided into control group of eight weeks(C8) and control group of 16weeks(C16);group M was divided into model group of eight weeks(M8)?model group of 16 weeks(M16) and exercise model group(EM). Group C feed the basic diet, group M was given high-fat diet for 8 weeks in a rat model of NAFLD. After 8 weeks, the rats of group C8 and M8 fasted for 12 hours were killed. After HE staining liver tissue pathology was observed under light microscope, detect the contents of blood TG,TC, AST, ALT, to evaluate the success rate of NAFLD rat model; begin from the ninth weeks, Group EM engaged in a period of eight weeks(60min /day, 6 days/ week) no load swimming training. After 16 weeks training, The rats of group C18?M18 and EM were killed.Then detect the contents of blood TG, TC, AST, ALT, take liver tissue ?testicular tissue and fat and weigh,record,keeping them respectively, after HE staining liver tissue and testicular tissue pathology were observed under light microscope;TUNEL method is used to mark testicular germ cell apoptosis, Calculate the average optical density value; use Western blot method to detect the expression of testicular tissue Fas/Fas L, Caspase-3 proteins in each group. Results:(1) Rats in group C8 did not appear obvious hepatocyte fatty change,eight rats in group M8 had fatty change,compare to C8,M8 was significantly increased in the integral of hepatocyte fattychange degree. Two rats in group C16 showed a small amount of hepatocyte fatty change; group M16 rats all have significant hepatocyte fatty change, six rats in group EM(n=9)showed fatty change. The degree of hepatocyte fatty change integral M16 and EM were significantly higher than that of C16,EM group was significantly lower than that of M16.(2) Compared to C8, serum AST, ALT, TC, TG of M8 were significantly increased; compared to C16, serum AST, ALT, TC, TG of M16 were significantly increased, TC level of group EM increased significantly, although the AST,ALT, TG content increased, there is no statistical difference between groups.(3) Fat weight around testicular in group M16 and EM showed mild rising and falling respectively than that of C16, the index of testicular in group M16 and EM showed mild falling and rising respectively than that of C16, but had no statistical difference between groups; the index of testicular in group EM increased significantly than that of M16.(4)Seminiferous tubules of rats in M16 loose arrangement, spermatogenesis,malalignment, representing the difference between group C16; seminiferous tubule of rats in EM tightly packed than M16, spermatogenesis, arrangement no obvious abnormalities, significantly better than M16.(5) Compared to group C16, testicular germ cell apoptosis and expression of Fas/Fas L,Caspase-3 proteins in group M16 were significantly increased while group EM had a mild increase;testicular germ cell apoptosis and expression of Fas/Fas L,Caspase-3 proteins in group EM were significantly decreased than that of M16.Conclusions:(1)High fat diet induced the formation of NAFLD in SD rat and with the extension of feeding time, the degree of NAFLD gradually deeper.(2)The number of NAFLD rat testicular germ cell apoptosis increased, and Fas / Fas L, Caspase-3 protein content of testicular tissue increased is an important reason.(3)Eight weeks swimming significantly reduce the degree of NAFLD in rats, while reducing the degree of testicular germ cell apoptosis significantly, lower the expression of Fas / Fas L, Caspase-3 proteins maybe is one of the important mechanisms.