Determination Of Synthetic Pigment In Food

Food pigment is a kind of common food additives. It is widely applied in the food industry. However, some businessmen who had been driven by the huge economic benefits used the excess or ultra range synthetic pigment in order to make the food beautiful. It poses a great threat to food safety. So the kinds and usage of the synthetic pigment are ruled strictly in the world.The detection methods of the food pigment were established by optimization of determination conditions for the high performance liquid chromatography(HPLC). And the pigment was detected in beverage and puffed food. The foundation was laid for the determination of the food pigment. The main researches were as follows.The determination method of food pigment was established by HPLC. The material were four food pigments for carmine, Amaranth, sunset yellow and lemon yellow in beverage and Puffed food. The chromatographic column was Agilent XDB-C18 column(4.6 mm×250 mm, 5 μm).The mobile phase was methanol-0.02 mol/L ammonium acetate.The elution conditions were : flow rates was 0.80 mL/min, the detection wavelength was254 nm, the concentrations of methanol were 20%~50%, 50%~75% and 75%~20% when the times were 0~9 min, 9~18 min and 18~20 min respectively. The method was simple and applicable to rapid determination of the synthetic pigment in beverage and puffed food.The food pigment was determinated in the sample. The samples were Mirinda, Fanta and puffed food. The carmine, Amaranth, sunset yellow and lemon yellow were determinated in the samples by the above method. So the content of pigments in sample may be confirmed whether it was in accord with the national standard.