Isolation Of Polyphenols From The Root Of Rhodomyrtus Tomentosa (Ait.) Hassk And Its Quality Control Methods

Rhodomyrtus tomentosa (Ait.) Hassk is a flowering plant in the family Myrtaceae and often be used in traditional Chinese medicine. In this paper, high performance liquid chromatography (HPLC) was used to analysis the chemical constituents of the root of Rhodomyrtus tomentosa, and the semi-preparative liquid chromatography was used to separate the components.Using the UV detector, the main compounds were separated by the semi-preparative liquid chromatography and the chemical structures were established on the basis of 13C-NMR,1H-NMR and MS. The content of gallic acid in the root of Rhodomyrtus tomentosa was measured by HPLC method. The HPLC gradient elute method was used to establish the figerprint of the root of Rhodomyrtus tomentosa and a software "Similarity evaluation system for chromatographic fingerprint of TCM(Version 2004 A)" was used for matching and similarity evaluation of the relevant parameters of the chromatograms of the root of Rhodomyrtus tomentosa from 10 different origin.As a result, four compounds comprising gallic acid, ellagic acid,3-methyl ellagic acid and 3,4,3’-trimethyl ellagic acid were separated from the root of Rhodomyrtus tomentosa. And the figerprint was established by HPLC method under the condition of the wavelength was 260nm and gradient elute. Gallic acid as a reference peak, The HPLC figerprint of the 10 batches of the root of Rhodomyrtus tomentosa in 90 min had been established and they was found 22 common peaks.The content of gallic acid was measured by HPLC at the wavelength of 270 nm and the retention time at about 10 min. It is simple and efficient to separate chemicals from natural products by the semi-preparative liquid chromatography. The average content of gallic acid in different batches of the the root of Rhodomyrtus tomentosa was determined by the HPLC. The HPLC for determination the content of gallic acid had high sensitivity, specificity and good reproducibility and it could measure the gallic acid content in the root of Rhodomyrtus tomentosa quickly and accurately. The figerprint method had better stability, precision and reproducibility. Anyway, because of the figerprint of the root of Rhodomyrtus tomentosa from different origin with high similarity and pertinency, using for research and quality assessment of the root of Rhodomyrtus tomentosa was feasible.