| Angelica sinensis the Μmbelliferae Angelica perennial herbaceoμs plants， ofwhich the dry storage root is commonly used in traditional Chinese medicine with thefunction of enriching the blood，promoting blood circμlation and regulating themenstrual fμnction. For a long time，there exists several problems in Angelica sinensisplanting process，such as "must raw land seedling culture" and "cultural barrierscontinuoμs Cropping Stress". In recent years，with the increasing of Angelica sinensisdemand and the expansion of planting area，and the shortening of the period of rotation，Angelica root rot is becoming more and more serious year by year，which seriouslyaffected the Chinese Angelica sinensis cultivation and sustainable development ofrelevant industries. At present the study of Angelica sinensis mainly focuses onpharmacology，cultivation，anatomy，etc.，as for the problem of Angelica sinensisinfected with root rot and the prevention of angelica root rot in the actual plantingprocess on plant physiology，it has not been reported yet. Therefore，this research usingAngelica sinensis seeds，seedlings and plants as materials，makes a study of theinfluence of root rot fungus on Angelica sinensis seed germination. After putting someinjuring to the root of seeding， the author inoculates with different strains of angelicaroot rot so as to measure the indexes of seedling growth and its’ physiological indexes，to obtain the influence of root rot fungus on Angelica sinensis seedling growth andphysiological. At last，the healthy Angelica sinensis are inoculated artificially withdifferent strains of angelica root rot. And the changes of10indexes are measured beforeand after the inoculation of7d，14d，21d，28d， including superoxide dismutase(SOD)，peroxidase (POD)， catalase (CAT)，hydrogen peroxide (H2O2)，malondialdehyde (MDA)，soluble protein，soluble sugar，proline and chlorophyllcontent， so as to study the dynamic change regulation about the physiological andbiochemical indexes and know the physiological mechanism of Angelica sinensisinfected with root rot fungus，which provides the theoretical basis to comprehensive andeffective prevention and control of Angelica sinensis root rot. The main results are as follows:1. It shows that the germination rate, germination potential, germination index andvigor index of Angelica sinensis seeds are decreased compared to the controls afterbeing infected with different strains of angelica root rot in the process. The germinationpotential is decreased by13.26℅,17.34℅after the seeds being infected withJQ086547(N6) and the mixed fungus, which means a much significant differencecompared with the control group(P <0.05). Among the rest of the index, there are nosignificant differences from the control, neither from the different treatments. Theheight, root length and fresh weight of Angelica sinensis seedling were obviouslyinhibited after being inoculated with angelica root rot, among which the seedling growthindex has a significant difference form the controlling ones after the injuring of blendtreatment.2. When Angelica sinensis are inoculated with root rot fungus, seedling SOD, POD,CAT activity are higher than the controlling ones, which means a significant differencebetween them(P <0.05). In all treatments, there is a significant difference (P <0.05)between the blend fungus and N8, a lower significant difference from N6. Solubleprotein content and relative conductivity of the seedlings are significantly higher thanthe control group, with a significantly difference in the treatments with the controlgroup. It shows that the physiological metabolism of seedling has changed and causedsome damage to the growth of Angelica sinensis seedlings after inoculated with root rotfungus.3. When Angelica sinensis are inoculated with different strains of root rot, theactivities of SOD, POD and CAT increase first but then decline along with the passingof the inoculation time, at7days SOD and CAT reach its’ peak respectively, theactivity of POD is also significantly increased and reach a peak at14days, after that allof the three have the declining tendency, which shows an extremely significantdifference (P <0.01) compared with before the inoculation and the control. It showsthat the plant physiological metabolism has caused serious influence and very hurt thegrowth of Angelica sinensis plant after inoculated with root rot fungus.4. The degree of Angelica sinensis cell membrane lipid peroxidation is enhancedafter inoculated with root rot fungus. the content of H2O2and MDA show a rising trendwith the extension of inoculation time, and a bigger increasing speed after14days. Andthe content of H2O2and MDA at21d are obviously higher than that at14d, and reach the maximum at28d which means has a extremely significant difference compared withbefore the inoculation and the control (P <0.05), among which the content of H2O2andMDA injured by the blend fungus are the highest.5. The tendency of the content of soluble protein and soluble sugar are similar tothe activities of antioxidant enzyme, but soluble sugar has a rapidly increasing within7days and reach it’s peak at the7th days, while soluble protein reaches the peak at14d,showing a significant difference compared with before the inoculation and the control(P <0.05). The content of proline continuously increasing tendency with the treatingtime going by.6. After Angelica sinensis being inoculated with different root rot fungus, thecontent of chlorophyll shows a decreasing trend with the extension of inoculation time.At28d, the content of chlorophyll in infected plants inoculated with varioμs funguscompared with the control, showing an extremely significant difference (P <0.01). |
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