Study On The Regularity Of Hair Follicle Development And The Expression Ofβ-catenin And BMP2in Skin Of Young Minks

The study was designed to investigate the development regularity of skin and hair follicle, the mRNA expression and protein localization of β-catenin and BMP in the skin of young American shorthair black male minks. To investigated the important role of β-catenin and BMP2in growth and development of skin and hair follicle of young minks. To provided some basic data about the morphological of hair follicle development in mink for further mechanisms study. To provided experimental basis for expression and function analysis of β-catenin and BMP2in mink skin and hair follicles. Those can help us to clearly understand the variation mechanism of mink hair follicle development and periodic.1-The study investigated the structure and development traits of skin and hair follicle on0to6weeks of minks. The dorsocentral region of skin samples were collected from three minks per week. Tissue sections were used to frozen section technique and HE staining method. The results showed that,1) The epidermis of the young minks significantly thickened at2weeks old, then became thin and kept stable. The dermal of the minks thickened from0to3weeks old, then kept stable;2) The guard hair and underfur of minks had hair medulla;3) The structure of the primary hair follicles had formed before mink borned. The density of the primary hair follicles first increased and then decreased with weeks old increasing. Mink borned without the secondary hair follicles, but at4week old, some of the secondary hair follicles had formed. The density of the secondary hair follicles increased with weeks old increasing. Secondary hair follicles and primary hair follicles average ratio (S/P) is2.95. The primary hair follicles and the secondary hair follicles deepened with weeks old increasing. We could conclude that primary hair follicles in the embryo have already formed and no longer developed or rarely developed after4week old. Secondary hair follicles occurred at4week old. Secondary hair follicles still develop after6week old.2-This experiment was conducted to investigate the location of (3-catenin and BMP2protein in skin from0to6weeks old of minks, by using the frozen section technique and immunohistochemistry analysis. The results showed that both β-catenin and BMP2expressed in the epidermis and outer root sheath cells of hair follicles. P-catenin and BMP2protein were always expressed in the development process of skin. β-catenin protein was almost expression on outer root sheath cells of hair follicles in early skin development. With the weeks old increasing, parts of the hair follicles β-catenin protein expressed weakly or even not expressed. BMP2protein expressed partly and weakly in the hair follicles from0to6weeks old of minks. We could conclud that β-catenin was abundantly expressed in the early developing hair follicle, with the hair follicle maturing, expression became weak. B-catenin may be involved in the regulation of skin and hair follicles development of young mink. The protein expression of BMP2may be involved in the impact of cycle changes in the hair follicle.3.The mRNA expression of β-catenin and BMP2in the skin from0to6weeks old of minks was studied by quantitative PCR comparative CT (AACT) experiment. The dorsocentral region of skin samples were collected from three minks per week. The results showed that β-catenin strongly up-regulated at4weeks old and reached the maximum at5weeks old, then decreased at6weeks old, while the expression of BMP2mRNA kept stable with weeks old increasing. Combined the results of hair follicle histology of young mink we can conclude that β-catenin plays a key role in the5-week-old mink hair follicle. It may be involved the development of secondary hair follicle in mink, BMP2does not have a direct impact for hair follicles of mink of0-6week old.