Test Flock Management And Subsidiary Job For Transgenic Studies Of Shaanbei Cashmere Goats

In order to improve the cashmere and meat production performance of Shaanbei White Cashmere Goats, we used CRISPR / Cas9 technology and transgenic somatic cell nuclear transfer to carry out research on Shaanbei White Cashmere Goats with the support of the development of new gene modification breeds. The study mainly focus on gene modification of cashmere and meat quality with the aim to get a stable genetic northern Shaanbei White Cashmere Goat strain group with high-quality cashmere and meat and propogate elite group lines.Besides the key technology mentioned above, superovulation, breeding, embryo collection of the donor ewes, estrus synchronization and embryo transferring of recipients, pregnancy management, delivering and caesareans of pregnant ewes, nursing management, breeding, growth monitoring, characters monitoring of lamb are of vital significance during the study. These work complement with laboratory work, and doing these work well can not only improve the efficiency of the entire project, but also ensure and guarantee gene modification traits related studies going well.During the preparation phase of Northern Shaanxi cashmere goats gene modification traits, we carried out a series of work, including number, epidemic prevention, insect repellent, body condition assessment, the donor ewe selection, estrus identification of the goat et.al. The study found that goat’s estrus cycle is 16-22 days, averages 19~20 days and test goat estrous duration for 24 to 48 hours.According to the estrous cycle, selecting the estrous cycle in the first 4-6 days(onset of estrus as day 0) as the donor, then the CIDR was used, On the tenth day after CIDR treatment intramuscular injection Folltropin-V 3.5 mL at 7 am, then Folltropin-V 2.5 mL at 7 pm. At day of 11, intramuscular injection Folltropin-V 2.0 mL at 7 am, then Folltropin-V 2.0 mL at 7 pm. At day of 12, intramuscular injection Folltropin-V 1.5 mL at 7 am, then Folltropin-V 1.0 mL in combine with PG 1.0 mL at 7 pm and withdraw CIDR. The result showed 82 super donor ewes all rutting and 79 of them were mated. We recycled 936 embryos and the number of viable embryos were up to 862.After gene editing with CRISPR/Cas9, we got 416 embryos and the rate of embryo division reached to 48.25%.We treated recipients with NRID at the 4 ~ 6 days of estrous cycle, and intramuscular injection FSH at 7 am and 7 pm at the day of 14~16 of estrous cycle. The recipients could be divided into three groups by different dose of FSH, the first group with FSH 33 IU in 76 recipients, the second with FSH 50 IU in 72 recipients, and the third with FSH 75 IU in 63 recipients. We withdraw the sponge at 7 am on 17 days after estrus and intramuscular injection PG 0.1mg. The result indicated that there was no difference in the rate of synchronization estrus(P> 0.05), however, the number of egg in FSH50 group higher than the other two groups(P <0.05).We got 93 CRISPR / Cas9 technology goats after delivery and cesarean section. We determinated body weight and body size of lamb, and the result demonstated that growth and development of transgenic goat more quickly than control ones. Transgenic nuclear transfer experiment obtained 3461 nuclear transfer cleavage embryo and transplant 183 receptors. 58 of those receptors is not estrus, 7 recptors aborted eight fetuses, 24 receptors were full-term delivery or caesarean and 25 lambed.