| There are abundant resources of Eucommia ulmoides Olive in China. It contains many kinds of components like lignans, iridoids, flavonoids, phenylpropanoids, with a wide range of applications to cure many diseases. In recent years, due to the lack of standardized research, caused a serious waste of Eucommia resource. With the rapid development of modern knowledge-based economy, using advanced information technology to strengthen the construction of Eucommia ulmoides chemical composition information database, accelerating information sharing and utilization, quickly updating. structure information, identification information, testing information and biological activity information of Eucommia. have become the trend of modernization.This paper established an information database of the chemical composition of Eucommia ulmoides Olive. The computer simulation, data mining and other advanced technology were integrated into the in-depth study of Eucommia ulmoides. Modern separation and identification techniques were used to isolate, purify and identify the chemical components of Eucommia ulmoides. These provided adequate informations and materials for further researches on network pharmacology, active screening and mechanism. A quantitative method using high performance liquid chromatography was established to simultaneously determine six active ingredients in Eucommiae Folium. Results of the study were provided a convenient method for the rapid detection of optimal harvest Eucommia leaves and a variety of active ingredients. Main contents were as follows:(1) The Eucommia ulmoides compounds database(EUCD) was built by the database management system of ISIS/Base. The database consisted of the information of 102 compounds of Eucommia ulmoides, including 19 items of each compounds on structure information, identification information, testing information, biological activity and references, providing a research platform for the relevant researchers. Use of information in the database can be quickly identified isolated compounds improved test efficiency. In the meantime, the database is supposed to be convenient for further study.(2) Eleven compounds were isolated from the ethanol extracts of the bark of Eucommia ulmoides Olive. Various chromatographic techniques, i.e. silica gel, polyamide, AB-8 macroporous resin, PTLC, MIC, C18 reversed-phase Silica gel, Sephadex LH-20 and HPLC were used in the process of isolation and purification. They were elucidated on the basis of comprehensive spectroscopic methods, such as UV, IR, 1H-NMR and 13C-NMR, etc. They were identified as betulinic acid(EUE-1), tetracosanoic acid(EUE-2), daucosterol(EUE-3), quercetin(EUE-4), aucubin(EUE-5), rutin(EUE-6), betulin(EUE-7), ferulic aldehyde(EUE-8), geniposide(EUB-1), geniposidic acid(EUB-2), syringaresinol-4’-O-D-Gluco-pyranoside(EUB-3).(3) Different coppice Eucommia leaf harvest season acquisition of six active ingredients was determied by using high performance liquid chromatography. A Thermo BDS HYPERSIL C18 chromatographic column(4.6 mm × 250 mm, 5 μm) was used to perform the determination, which was maintained at 35 ℃ throughout the analysis. Mobile phase was composed of methanol(A)- 0.05% phosphoric acid water solution(B) with flow rate at 0.8 m L/min under gradient elution by programmed wavelength detection. Results also showed that the contents of aucubin, geniposidic acid and chlorogenic acid had the evident differences in different periods. The HPLC detection method has a good reproducibility and maneuverability. This method is sensitive and suitable to investigate the ingredients differences in coppiced Eucommiae Folium. As a result, suitable harvesting time for Eucommiae Folium from Eucommia Coppice is August. |
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