The Study Of Development Of Mast Cells And Lymphoid Tissue In The Chicken Oviduct

The cloaca is the organ that has direct exposure to the environment for laying hens. Antigens are able to invade the oviduct through cloaca with ease. In a situation like this, mucosal-associated lymphoid tissue is the first line of defense against pathogens in the oviduct. The oviducts were sampled from laying hens over the course of study on day 1,day 4,day 7,day 14, day 21,day 35,day 56,day 90,day 140,day 210, day 280, and day 350. Part of the tissue materials were made into paraffin section and stained with toluidine blue. Then the change in regulation of mast cells development was further analyzed by histological research methodologies. The rest of the samples were made into frozen sections, in order to follow the appearance, migration, location and quantity of T lymphocytes cells and B lymphocytes cells in oviduct of laying hens by immunohistochemistry. This enables further understanding of the characteristics of lymphoid tissue of oviduct during different periods, and providing theoretical basis for the prevention and treatment of diseases of the reproductive tract of laying hens.The development of mast cells during different periods of oviduct was scrutinized by toluidine blue staining. The results showed that the mast cells were first observed at day 56 in the oviduct, the number of mast cells was reached the peak in the unit vision at day 210. Lots of mast cells were located in subepithelium, amina propria, and muscular, around the blood vessels. The majority of mast cells in the unit vision of infundibulum, while the cell of isthmus was the least abundant in the oviduct.The development of T lymphocytes cells and B lymphocytes cells in oviduct was observed by immunohistochemistry. The results showed that T lymphocytes cells were the first appeared at day 35 in the oviduct, and the number of cells reached the peak at day 140, then they were reduced afterwards. Overall, the number of T lymphocytes cells was most abundant in vagina, compared with other parts of oviduct. The aggregation of T cells was found in the oviduct at day 140, while less aggregation was found on day 210. Scattered lymphocytes were mainly presented at other days. Bu-1+cells were first appeared at day 21, reaching the peak at day 140.The results showed that the mast cells reached maximum at day 210, mainly located in subepithelium, amina propria, and muscles. CD3+cells, CD4+cells, CD8+cells and Bu-1+cells were mainly located in epithele and lamina propria, reaching the peak at day 140.