The Influence Of Main Types Cells And Cyclin D1, P27 MRNA Expression In Blood Of Chicks Infected With REV

Reticuloendotheliosis(RE) was an immune suppression of tumor disease of chicks by retieuloendotheliosis virus(REV). One day old SPF chicks were used in this study. Wright’s stain, Esterase staining, the technologies of MTT, ELISA, realtime fluorescence quantitative PCR were used for revealing the number of white cell, heterophilic granulocytes, TANAE+ cell, the content of Ig S(IgA, Ig G, Ig M), the proliferative function of T and B lymphocytes, the dynamic change of Cyclin D1, p27 mRNA expression. Aimed at further revealed the influence of immunity ability and Cyclin D1, p27 mRNA expression in peripheral blood of chicks infected with REV. To provide important scientific basis for prevention and treatment of RE. The results were as follows:(1)One day old SPF chicks infected with REV, the number of white cells,heterophilic granulocytes, TANAE+ and the proliferative function of T lymphocytes in peripheral blood of chicks were lower than that of control group at 1~42 days; the number of heterophilic granulocytes, TANAE+ of infected chicks were significantly lower than that of control group at 14~42 days(P<0.01); the number of white blood cells of infected chicks were significantly lower than that of control group at 7~42 days s(P<0.01); the proliferative function of T lymphocytes in peripheral blood of chicks were significantly lower than that of control group at 14~21 days and 42 day(P<0.01 or P<0.05). These result indicated that the function of cellular immunity in peripheral blood of chickens infected with REV was reduced.(2)One day old SPF chicks infected with REV, the proliferative function of B lymphocytes and the content of Ig A, Ig G, Ig M in peripheral blood of chicks were lower than that of control group at 1~42 days; the content of Ig A, IgG, Ig M in peripheral blood of chicks were lower than that of control group at 21~42 days, 14~28 days, 42 days(P<0.01 or P<0.05); These result indicated that the function of humoral immunity in peripheral blood of chickens infected with REV was reduced.(3)One day old SPF chicks infected with REV, the expression of Cyclin D1, p27 mRNA in peripheral blood of chicks were higher than that of control group at 7~42 days, the expression of Cyclin D1 mRNA were higher than that of control group(P<0.01). These result indicated that the overexpression of Cyclin D1, p27 mRNA wereclosely related with cell proliferation by virus infected.