Study On Technique Of Rapid Propagation Of Pepper(Capsicum Annuum L.)through Tissue Culture

There are still many shortcomings in the application of tissue culture and rapid propagation technology to seedling nursery of pepper. In this thesis, factors influencing each stage of tissue culture of pepper were investigated and the rapid propagation technique of pepper was optimized. The main results were as followings.1. The sterilization methods of seeds and hypocotyls were studied, results showed that, the best sterilization method for pepper seeds was 0.1% mercuric chloride 9 min; The best sterilization method for hypocotyls was 0.15% mercuric chloride 7 min followed by sterile water washing 6 times.2. Effects of seedling age and explants types on shoot induction were studies,results showed that, seedlings of 15 d shoed the shortest budding time, most nuber of buds inducted, and highest induction rate; best results could be obtained with length of hypocotyls 0.5 ~ 1 cm.3. Effects of different plant growth regulators on bud induction were studied,results showed that, 2,4-D induced callus instead of bud from leaf or hypocotyls; The best combination of PGRs for bud induction was 6-BA 3 mg / L+ NAA 0.8 mg / L;The best results of bud induction with TDZ was obtained at concentration of 1 mg / L.4. Effects of plant growth regulators on bud proliferation were studied, results showed that, the best concentration of TDZ for proliferation of single bud was 1 mg /L; GA3 enhanced elongation of buds of type A and B; the best combination of PGRs for bud proliferation was 6-BA 3 mg / L + NAA 0.8 mg / L + GA3 1.5 mg / L; KT at 4mg / L showed the largest effects of elongation on bud.5, Effects of plant height and plant growth regulators rooting of buds were studied, results showed that, IBA at concentration of 0.9 mg / L was best for rooting;the best medium for rooting was 1 / 2 MS + IBA 0.7 mg / L.