| The study was conducted to investigate the effects of in ovo feeding of disaccharide on growth performance and intestinal development prior in Landaise geese. A total of 300 hatching eggs of Landaise geese were randomly divided into 2 treatments(Control and Disaccharide) with 3 replicates for each treatment, and 50 eggs for each replicate. At 24 d of incubation, fertile eggs were injected with 1.5 mL of 25 maltose g/L, 25 sucrose in 0.75% saline. The effects of in ovo feeding disaccharide on Landaise geese were determined by the intestinal weight, mucosal morphology, growth performance, intestinal disaccharide enzyme activities and mRNA expression.The results showed that the intestinal development after the first day of hatch were significantly higher than that at E23(P < 0.05), after 24 h of hatching the depth of the duodenum, jejunum and ileum were significantly increased(P < 0.05) by 30.29%, 120.27% and 89.41% respectively.At 28 d, the results were significantly(P<0.05) higher than the first day, whereas the weight of duodenum, jejunum and ileum were improved 19.07, 24.14 and 23.45 times respectively than the first day hatching results. The effects of in ovo feeding disaccharide on the jejunum were according with the small intestine. For treated group it was found that H/C increased 20.57%(P<0.05), villi width increased 16.11%, in ovo feeding disaccharide made H/C increased 1.46 times than control(P<0.05)1d. In ovo feeding of disaccharide increase H/C in 34.19% and villi width in 12.26%(P<0.05).After two weeks of incubation the crypt depth of injection group decreased 16.84% and villi weight increased 12.26%;21d, in ovo feeding of disaccharide increased villi hight 19.18%, the weight of the jejunum was 25.13 times than control group,as that increase,at 28 d.Regarding to the effects of disaccharide supplies on the embryo, it could improve the level of Landaise geese average daily gain, comparing with the control group, the average daily gain increased 16.32%(P < 0.05).At the period from 1~14d, while reducing the weight ratio of the Landaise geese. The rate decreased to be 5.65% as(P<0.05)a result to in ovo feeding disaccharides for the average daily gain at 15~28d.The effects of in ovo feeding of disaccharides on jejunum, and the effect of disaccharides on the expression of transport mRNA: The jejunal sucrase increased 49.01%,which exhibited a tendency towards the higher activities(0.05≤P<0.10). Compared with the control group, at the seventh day the injection group was 22.33% higher(P < 0.05) than control. At the end of the expression, the activity of maltase in injection group was 1.24 times than control group, which showed a higher tendency(0.05 ≤P <0.10).Enhancing the expression of the jejunal SI,SGLT1 and GLUT2 were found to be 42.05%,77.31% and 81.48% respectively, at 7d for the disaccharides solution treated group in comparison with the control group. In conclusion, in ovo feeding disaccharides may promote the Landaise geese growth performance, for the small intestine growth, morphology of intestine mucosal, and improve the jejunum disaccharides activity and expression of disaccharide transport genes mRNA.Therefore, in ovo feeding disaccharides was enhancer to the development of small intestine,promoted the nutrient uptake, and improved the hatchlings quality of Landaise geese. |
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