Preventive Effect Of Codonopsis Lanceolata On Diethylnitrosamine Induced Liver And Colon Injurey

ObjectiveCodonopsis lanceolata is a Campanulaceae Codonopsis species. Modern studies suggest that it has a certain antioxidant, anti-aging, anti-mutation effect, at the same time it could prevent the liver injury of various causes. Diethylnitrosamine (DEN) has proved to be the cancer inducing agent, significant effect in liver cancer in particular. To study prevention effect of codonopsis lanceolata on diethylnitrosamine induced liver and colon injurey, it provides a new theoretical basis for the development of anticancer drugs.Methods60 Kunming female mice, which level were the general, were selected from the Laboratory Animal Department of Yanbian University. According to the weight of the mice, they were divided into 5 groups randomly, which were normal control group, model group, and Codonopsis lanceolata extract treated group, which subdivided into low-dose group, middle dose group and high dose group based on the gavage dose of Codonopsis lanceolata extract. In each group,there were 12 mice. By intraperitoneal injection of DEN (given by 25 mg/kg, once/week, for 8 weeks), establishing injury model of liver and colon. At the same time, all of the groups were given fermentation of Codonopsis lanceolata (fermenting Codonopsis lanceolata with active yeast, and obtained fermented Codonopsis lanceolata extract after ethanol extraction and butanol extraction), on the basis of 0.1 ml/10g.And normal control group and the DEN group were given normal saline, low-dose group, middle dose group and high dose group were given fermentation of Codonopsis lanceolata,which does were 1 g/kg,2 g/kg,4 g/kg in order for 8 weeks. Fasting 12h after the last gavage, all the mice were cervical dislocation to 8 week, then removed their liver and colon quickly, which were made into 10% homogenate with cold homogenization medium. Detecting COX-1, COX-2, NF-κB level of liver and colon tissue by ELISA assay, and measuring the levels of NO, iNOS, TNOS of the liver and colon tissue with colorimetric. The liver and colon specimens were stained with hematoxylin eosin, and observed their pathological changes.Results1. Codonopsis lanceolata extract treatment group compared with the model group, the level of COX-1, COX-2, NF-κB protein expression of the liver tissue and the colon tissue was significantly decreased, and its role showed dose dependent.2. Codonopsis lanceolata extract treatment group compared with the model group, iNOS, TNOS activity was inhibited, and the expression of NO protein was decreased, and its role also showed dose dependent. And there were significant differences in each concentration group.3. Observing pathologic specimen, there is a large number of liver cell degeneration and necrosis, and the liver damage of the treatment group less severe than the model group.Conclusion1. Fermented Codonopsis lanceolata extract can prevent liver and colon toxicity induced by DEN.2. The fermentation of Codonopsis lanceolata may be pass down the expression of NF-κB protein to inhibit the expression of COX-2 protein, and to inhibit the activity of iNOS, TNOS at the same time. As the activity of iNOS is decrease, a large number of synthetic of NO protein is inhibited to prevent the DEN induced the toxicity of liver and colon.