Influence Of Interleukin-1Receptor Antagonist Gene Polymorphism And Interleukin-1β Gene Polymorphism On The Primary Knee Osteoarthritis

Objective Osteoarthritis is a common chronic joint disease. The current viewis that OA may be caused by genetic factors, chronic fatigue, obesity and traumaticstress and other factors working together, can be tired and joints around the entireorganization recent studies have shown: Interleutin1(IL-1) is a core position in theinflammatory process is the most important cytokine-mediated articular carticulagedamage.Interleukin-1receptor antagonist(IL-1Ra),as a natural antagonist of IL-1,more and more attention.So to explore the relationship between the primary kneeostcoarthritis patients and interleukin-1,especially interleukin-1β(IL-1β) andinterleukin-1receptor antagonist(IL-1Ra)gene polymorphism.Methods Take in March2011to November2011NingXia medical universityhospital affiliated hospitals a primary knee osteoarthritis (n=120) and body health asthe control group (n=120). Extract the5ml of peripheral venous blood in all subjects,DNA was extracted,Polymorphisms in IL-1α and IL-1Ra were analyzed with PCR inorder to find the difference of the genotype distribution and the allele frequencybetween the PKOA patients and the healthy controls. SPSS12.0statistical software,statistical experimental group and control group with statistical significance.Results The genetype distribution and allele frequency of IL-lβ(+3953C/T)aresignificantly higher in statistics between the two group(P<0.05). While all kinds ofIL-1RN genotype frequenc ies of patients with PKOA didn’t differ from those of thecontrol(P>0.05). Conclusion Carrying the IL-1β+3954T/T genotype groups may increasesusceptibility to osteoarthritis; IL-1RN*2gene polymorphism may be associated withgenetic susceptibility of osteoarthritis is not relevant.