The Effect Of Ezrin Protein On The Growth And Invasiveness In Gastric Cancer

Objective To detect the expression of Ezrin protein in gastric cancerand research its association with the clinicopathologic features，andinvestigate its influence in prognosis. Explore the mechanism of theeffect of Ezrin protein on the growth and invasiveness in gastric cancer,preliminarily.Methods206cases of gastric cancer tissues without peritonealmetastasis,34cases of peritoneal metastatic tissues and54cases ofadjacent non-cancerous tissues paraffin-embedded specimens with detailedclinicopathological parameters were collected from Fujian MedicalUnivercity Union Hospital from December2006to September2007.Immunohistochemical SP method was used to detect the expression level ofEzrin and CD44v6.Western blot assay was used to detect the expressionlevel of Erin in16cases of gastric cancer tissues and its’adjacentnon-cancerous tissues were collected after operation in May to June of2012. The association of Ezrin expression with clinicopathologicalparameters and with CD44v6expression were analyzed by SPSS17.0softwareResults1.Ezrin protein can express in gastric cancer tissues, adjacentnon-cancerous tissues and peritoneal metastatic tissues, and mainly incytomembrane and cytoplasm. The rate of Ezrin protein expression ingastric cancer tissues was considerably higher than that in adjacentnon-cancerous tissues (P＜0.05). The rate of Ezrin protein expression inperitoneal metastatic tissues was considerably higher than that inprimary gastric cancer tissues(P＜0.05).2. Ezrin protein relativeexpression intensity in gastric cancer tissues (0.7962±0.4945) wassignificantly higher than its adjacent non-cancerous tissues (0.4355±0.4185), the difference was statisticly significant (P＜0.05).3. Cell function experiments showed that，the cell growth rate was significantlyinhibited (P＜0.05)and the cell invasion was significantly lower thancontrol group(P＜0.05),if we downregulate the expression of Ezrinprotein.4. The higher expression of Ezrin protein in gastric cancer wasassociated with lymphatic metastasis. Overexpression of Ezrin protein hada significant correlation with poor survival in prognostic analyses(P＜0.05).5. The rate of CD44v6expression in gastric cancer tissues wasconsiderably higher than that in adjacent non-cancerous tissues (P＜0.05). The expression of Ezrin protein and CD44v6was significantly positivelycorrelated(P＜0.05).Conclusion These results indicate that Ezrin protein was overexpressedin gastric cancer;downregulated expression of Ezrin protein contributedto inhibit the invasion and metastasis of gastric cancer; Ezrin wassignificantly associated with lymph node metastasis and prognosis; Ezrinprotein may affect the growth and invasiveness of gastric cancer throughCD44v6.